Seroatlas · Human Serome Atlas

ZNF324

Zinc finger protein 324A

Also known as: Z324A_HUMAN, ZF5128, ZNF324A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75467
Gene: ZNF324
Ensembl: ENSG00000083812
Chromosome: 19
Canonical length: 553 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Nuclear membrane,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within with a positive effect on G1/S transition of mitotic cell cycle and cell population proliferation. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

553 residues, UniProt reviewed canonical sequence.

>O75467|ZNF324
     1  MAFEDVAVYF SQEEWGLLDT AQRALYRRVM LDNFALVASL GLSTSRPRVV IQLERGEEPW
    61  VPSGTDTTLS RTTYRRRNPG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVAGACHSVK
   121  SLQRQRGASP SRERKPTGVS VIYWERLLLG SGSGQASVSL RLTSPLRPPE GVRLREKTLT
   181  EHALLGRQPR TPERQKPCAQ EVPGRTFGSA QDLEAAGGRG HHRMGAVWQE PHRLLGGQEP
   241  STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECAQC GKAFSQTSHL
   301  TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR
   361  KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFKHQRVHT
   421  GEKPFACPQC GRAFSHSSNL TQHQLLHTGE RPFRCVDCGK AFAKGAVLLS HRRIHTGEKP
   481  FVCTQCGRAF RERPALFHHQ RIHTGEKTVR RSRASLHPQA RSVAGASSEG APAKETEPTP
   541  ASGPAAVSQP AEV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF324 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 7.3 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 7.3 nTPM
cerebral cortex: 6.6 nTPM
pituitary gland: 6 nTPM
skin: 5.6 nTPM
esophagus: 5.1 nTPM
liver: 5.1 nTPM

Single-cell type

retinal amacrine cells: 25 nCPM
early primary spermatocytes: 25 nCPM
rod photoreceptor cells: 24 nCPM
tuft cells: 23 nCPM
breast lactating cells: 23 nCPM
retinal bipolar cells: 21 nCPM

Immune cell

neutrophil: 11 nTPM
naive CD4 T-cell: 8.1 nTPM
memory CD8 T-cell: 7.9 nTPM
MAIT T-cell: 7.8 nTPM
gdT-cell: 7.1 nTPM
naive CD8 T-cell: 6.8 nTPM

Brain region

cerebellum: 18 nTPM
cerebral cortex: 15 nTPM
basal ganglia: 14 nTPM
hippocampal formation: 14 nTPM
white matter: 13 nTPM
amygdala: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.61
gnomAD pLI: 0.04
gnomAD missense Z: 1.03
DepMap mean gene effect: -0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZNF324 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

TRIM28

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF324 as an antibody target. Whether an autoantibody or antibody against ZNF324 could matter depends on whether native ZNF324 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF324 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF324 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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