Seroatlas · Human Serome Atlas

ZFHX4

Zinc finger homeobox protein 4

Also known as: FLJ20980, ZFH4, ZFHX4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86UP3
Gene: ZFHX4
Ensembl: ENSG00000091656
Chromosome: 8
Canonical length: 3567 aa
Protein class: Disease related genes, Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

3567 residues, UniProt reviewed canonical sequence.

>Q86UP3|ZFHX4
     1  METCDSPPIS RQENGQSTSK LCGTTQLDNE VPEKVAGMEP DRENSSTDDN LKTDERKSEA
    61  LLGFSVENAA ATQVTSAKEI PCNECATSFP SLQKYMEHHC PNARLPVLKD DNESEISELE
   121  DSDVENLTGE IVYQPDGSAY IIEDSKESGQ NAQTGANSKL FSTAMFLDSL ASAGEKSDQS
   181  ASAPMSFYPQ IINTFHIASS LGKPFTADQA FPNTSALAGV GPVLHSFRVY DLRHKREKDY
   241  LTSDGSAKNS CVSKDVPNNV DLSKFDGCVS DGKRKPVLMC FLCKLSFGYI RSFVTHAVHD
   301  HRMTLNDEEQ KLLSNKCVSA IIQGIGKDKE PLISFLEPKK STSVYPHFST TNLIGPDPTF
   361  RGLWSAFHVE NGDSLPAGFA FLKGSASTSS SAEQPLGITQ MPKAEVNLGG LSSLVVNTPI
   421  TSVSLSHSSS ESSKMSESKD QENNCERPKE SNVLHPNGEC PVKSEPTEPG DEDEEDAYSN
   481  ELDDEEVLGE LTDSIGNKDF PLLNQSISPL SSSVLKFIEK GTSSSSATVS DDTEKKKQTA
   541  AVRASGSVAS NYGISGKDFA DASASKDSAT AAHPSEIARG DEDSSATPHQ HGFTPSTPGT
   601  PGPGGDGSPG SGIECPKCDT VLGSSRSLGG HMTMMHSRNS CKTLKCPKCN WHYKYQQTLE
   661  AHMKEKHPEP GGSCVYCKTG QPHPRLARGE SYTCGYKPFR CEVCNYSTTT KGNLSIHMQS
   721  DKHLNNVQNL QNGNGEQVFG HSAPAPNTSL SGCGTPSPSK PKQKPTWRCE VCDYETNVAR
   781  NLRIHMTSEK HMHNMMLLQQ NMKQIQHNLH LGLAPAEAEL YQYYLAQNIG LTGMKLENPA
   841  DPQLMINPFQ LDPATAAALA PGLGELSPYI SDPALKLFQC AVCNKFTSDS LEALSVHVSS
   901  ERSLPEEEWR AVIGDIYQCK LCNYNTQLKA NFQLHCKTDK HMQKYQLVAH IKEGGKSNEW
   961  RLKCIAIGNP VHLKCNACDY YTNSVDKLRL HTTNHRHEAA LKLYKHLQKQ EGAVNPESCY
  1021  YYCAVCDYTT KVKLNLVQHV RSVKHQQTEG LRKLQLHQQG LAPEEDNLSE IFFVKDCPPN
  1081  ELETASLGAR TCDDDLTEQH EEAEGAIKPT AVAEDDEKDT SERDNSEGKN SNKDSVSVAG
  1141  GTQPLLLAKE EDVATKRSKP TEDNKFCHEQ FYQCPYCNYN SRDQSRIQMH VLSQHSVQPV
  1201  ICCPLCQDVL SNKMHLQLHL THLHSVSPDC VEKLLMTVPV PDVMMPNSML LPAAASEKSE
  1261  RDTPAAVTAE GSGKYSGESP MDDKSMAGLE DSKANVEVKN EEQKPTKEPL EVSEWNKNSS
  1321  KDVKIPDTLQ DQLNEQQKRQ PLSVSDRHVY KYRCNHCSLA FKTMQKLQIH SQYHAIRAAT
  1381  MCNLCQRSFR TFQALKKHLE AGHPELSEAE LQQLYASLPV NGELWAESET MSQDDHGLEQ
  1441  EMEREYEVDH EGKASPVGSD SSSIPDDMGS EPKRTLPFRK GPNFTMEKFL DPSRPYKCTV
  1501  CKESFTQKNI LLVHYNSVSH LHKLKKVLQE ASSPVPQETN SNTDNKPYKC SICNVAYSQS
  1561  STLEIHMRSV LHQTKARAAK LEPSGHVAGG HSIAANVNSP GQGMLDSMSL AAVNSKDTHL
  1621  DAKELNKKQT PDLISAQPAH HPPQSPAQIQ MQLQHELQQQ AAFFQPQFLN PAFLPHFPMT
  1681  PEALLQFQQP QFLFPFYIPG TEFSLGPDLG LPGSATFGMP GMTGMAGSLL EDLKQQIQTQ
  1741  HHVGQTQLQI LQQQAQQYQA TQPQLQPQKQ QQQPPPPQQQ QQQQASKLLK QEQSNIVSAD
  1801  CQIMKDVPSY KEAEDISEKP EKPKQEFISE GEGLKEGKDT KKQKSLEPSI PPPRIASGAR
  1861  GNAAKALLEN FGFELVIQYN ENRQKVQKKG KSGEGENTDK LECGTCGKLF SNVLILKSHQ
  1921  EHVHGQFFPY AALEKFARQY REAYDKLYPI SPSSPETPPP PPPPPPLPPA PPQPSSMGPV
  1981  KIPNTVSTPL QAPPPTPPPP PPPPPPPPPP PPPPPPSAPP QVQLPVSLDL PLFPSIMMQP
  2041  VQHPALPPQL ALQLPQMDAL SADLTQLCQQ QLGLDPNFLR HSQFKRPRTR ITDDQLKILR
  2101  AYFDINNSPS EEQIQEMAEK SGLSQKVIKH WFRNTLFKER QRNKDSPYNF SNPPITVLED
  2161  IRIDPQPTSL EHYKSDASFS KRSSRTRFTD YQLRVLQDFF DTNAYPKDDE IEQLSTVLNL
  2221  PTRVIVVWFQ NARQKARKSY ENQAETKDNE KRELTNERYI RTSNMQYQCK KCNVVFPRIF
  2281  DLITHQKKQC YKDEDDDAQD ESQTEDSMDA TDQVVYKHCT VSGQTDAAKN AAAPAASSGS
  2341  GTSTPLIPSP KPEPEKTSPK PEYPAEKPKQ SDPSPPSQGT KPALPLASTS SDPPQASTAQ
  2401  PQPQPQPPKQ PQLIGRPPSA SQTPVPSSPL QISMTSLQNS LPPQLLQYQC DQCTVAFPTL
  2461  ELWQEHQHMH FLAAQNQFLH SPFLERPMDM PYMIFDPNNP LMTGQLLGSS LTQMPPQASS
  2521  SHTTAPTTVA ASLKRKLDDK EDNNCSEKEG GNSGEDQHRD KRLRTTITPE QLEILYEKYL
  2581  LDSNPTRKML DHIAREVGLK KRVVQVWFQN TRARERKGQF RAVGPAQSHK RCPFCRALFK
  2641  AKSALESHIR SRHWNEGKQA GYSLPPSPLI STEDGGESPQ KYIYFDYPSL PLTKIDLSSE
  2701  NELASTVSTP VSKTAELSPK NLLSPSSFKA ECSEDVENLN APPAEAGYDQ NKTDFDETSS
  2761  INTAISDATT GDEGNTEMES TTGSSGDVKP ALSPKEPKTL DTLPKPATTP TTEVCDDKFL
  2821  FSLTSPSIHF NDKDGDHDQS FYITDDPDDN ADRSETSSIA DPSSPNPFGS SNPFKSKSND
  2881  RPGHKRFRTQ MSNLQLKVLK ACFSDYRTPT MQECEMLGNE IGLPKRVVQV WFQNARAKEK
  2941  KFKINIGKPF MINQGGTEGT KPECTLCGVK YSARLSIRDH IFSKQHISKV RETVGSQLDR
  3001  EKDYLAPTTV RQLMAQQELD RIKKASDVLG LTVQQPGMMD SSSLHGISLP TAYPGLPGLP
  3061  PVLLPGMNGP SSLPGFPQNS NISAGMLGFP TSATSSPALS LSSAPTKPLL QTPPPPPPPP
  3121  PPPPSSSLSG QQTEQQNKES EKKQTKPNKV KKIKEEELEA TKPEKHPKKE EKISSALSVL
  3181  GKVVGETHVD PIQLQALQNA IAGDPASFIG GQFLPYFIPG FASYFTPQLP GTVQGGYFPP
  3241  VCGMESLFPY GPTMPQTLAG LSPGALLQQY QQYQQNLQES LQKQQKQQQE QQQKPVQAKT
  3301  SKVESDQPQN SNDASETKED KSTATESTKE EPQLESKSAD FSDTYVVPFV KYEFICRKCQ
  3361  MMFTDEDAAV NHQKSFCYFG QPLIDPQETV LRVPVSKYQC LACDVAISGN EALSQHLQSS
  3421  LHKEKTIKQA MRNAKEHVRL LPHSVCSPNP NTTSTSQSAA SSNNTYPHLS CFSMKSWPNI
  3481  LFQASARRAA SPPSSPPSLS LPSTVTSSLC STSGVQTSLP TESCSDESDS ELSQKLEDLD
  3541  NSLEVKAKPA SGLDGNFNSI RMDMFSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZFHX4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0
Highest tissue expression: 6.3 nTPM

Expression across tissuesHPA

Tissue

testis: 6.3 nTPM
seminal vesicle: 5.2 nTPM
adrenal gland: 3.3 nTPM
epididymis: 3.1 nTPM
colon: 3 nTPM
tongue: 2.9 nTPM

Single-cell type

pituitary stem cells: 476 nCPM
astrocytes: 406 nCPM
corticotrophs: 385 nCPM
adrenal cortex cells: 288 nCPM
oligodendrocyte progenitor cells: 281 nCPM
ependymal cells: 279 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

midbrain: 64 nTPM
hypothalamus: 54 nTPM
pons: 53 nTPM
thalamus: 47 nTPM
white matter: 45 nTPM
basal ganglia: 39 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ZFHX4.

Disease | GeneticClinVar

15 pathogenic / likely-pathogenic of 744 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Neurodevelopmental disorder
ZFHX4-related disorder
ZFHX4-associated neurodevelopmental disorder
Autosomal dominant ZFHX4-related disorders

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.14
gnomAD pLI: 1
gnomAD missense Z: 1.01
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of transcription by RNA polymerase II

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZFHX4 as an antibody target. Whether an autoantibody or antibody against ZFHX4 could matter depends on whether native ZFHX4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZFHX4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZFHX4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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