Seroatlas · Human Serome Atlas

WFDC9

Protein WFDC9

Also known as: dJ688G8.2, WAP9, WFDC9_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8NEX5
Gene
WFDC9
Ensembl
ENSG00000180205
Chromosome
20
Canonical length
89 aa
Protein class
Predicted secreted proteins
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

The WAP-type four-disulfide core (WFDC) domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many members of the WFDC domain family. This gene encodes a protein which contains a WFDC domain, and is thus a member of the WFDC domain family. This gene and several other gene family members are clustered at 20q13.12. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

89 residues, UniProt reviewed canonical sequence.

>Q8NEX5|WFDC9
     1  MKPWILLLVM FISGVVMLLP VLGSFWNKDP FLDMIRETEQ CWVQPPYKYC EKRCTKIMTC
    61  VRPNHTCCWT YCGNICLDNE EPLKSMLNP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WFDC9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
933 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 933 nTPM
  • seminal vesicle: 2 nTPM
  • adipose tissue: 0 nTPM
  • adrenal gland: 0 nTPM
  • amygdala: 0 nTPM
  • appendix: 0 nTPM

Single-cell type

  • epididymal principal cells: 1,412 nCPM
  • epididymal clear cells: 14 nCPM
  • epididymal basal cells: 13 nCPM
  • epicardial cells: 4.5 nCPM
  • mesothelial cells: 4.3 nCPM
  • mast cells: 3.1 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebral cortex: 0.3 nTPM
  • hippocampal formation: 0.2 nTPM
  • basal ganglia: 0.1 nTPM
  • hypothalamus: 0.1 nTPM
  • medulla oblongata: 0.1 nTPM
  • pons: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.76
gnomAD pLI
0.01
gnomAD missense Z
0.35
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WFDC9 as an antibody target. Whether an autoantibody or antibody against WFDC9 could matter depends on whether native WFDC9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WFDC9 is annotated as secreted, so native WFDC9 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label WFDC9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/WFDC9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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