WDFY4

Protein identityUniProt · HPA

UniProt accession

Q6ZS81

Gene

WDFY4

Ensembl

ENSG00000128815

Chromosome

10

Canonical length

3184 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to be involved in antigen processing and presentation. Predicted to act upstream of or within with a positive effect on CD8-positive, alpha-beta T cell activation. Predicted to act upstream of or within cellular response to virus. Predicted to be located in early endosome and endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

3184 residues, UniProt reviewed canonical sequence.

>Q6ZS81|WDFY4
     1  MEAEDLSKAE DRNEDPGSKN EGQLAAVQPD VPHGGQSSSP TALWDMLERK FLEYQQLTHK
    61  SPIERQKSLL SLLPLFLKAW EHSVGIICFP SLQRLAEDVS DQLAQQLQKA LVGKPAEQAR
   121  LAAGQLLWWK GDVDQDGYLL LKSVYVLTGT DSETLGRVAE SGLPALLLQC LYLFFVFPLD
   181  KDELLESDLQ VQKMFVQMLL NICSDSQGLE GLLSGSELQS LLIATTCLRE HSCCFWKEPT
   241  FCVLRAISKA QNLSIIQYLQ ATDCVRLSLQ NLSRLTDTLP APEVSEAVSL ILGFVKDSYP
   301  VSSALFLEFE NSEGYPLLLK VLLRYDGLTQ SEVDPHLEEL LGLVVWLTTC GRSELKVFDS
   361  ITYPQLEGFK FHHEASGVTV KNLQAFQVLQ NVFHKASDSV LCIQVLSVIR TMWAWNARNF
   421  FLLEWTLQPI SQFVEIMPLK PAPVQEHFFQ LLEALVFELH YVPHEILRKV QHLIKESPGP
   481  SCTLMALQSI LSIAGGDPLF TDIFRDSGLL GLLLAQLRKQ AKIMRKSGNK VSTPGVQDPE
   541  RELTCVMLRI VVTLLKGSVR NAVVLKDHGM VPFIKIFLDD ECYREASLSI LEQLSAINAE
   601  EYMSIIVGAL CSSTQGELQL KLDLLKSLLR ILVTPKGRAA FRVSSGFNGL LSLLSDLEGS
   661  LQEPPLQAWG AVSPRQTLEL VLYTLCAVSA ALHWDPVNGY FFRRNGLFEK LAEDLCLLGC
   721  FGALEEEGNL LRSWVDTKAR PFADLLGTAF SSSGSLPPRI QSCLQILGFL DSMASGTLHL
   781  RGDLKESLRT KQGPVVDVQK GETGSDPQRN FKQWPDLEER MDEGDAAIMH PGVVCIMVRL
   841  LPRLYHEDHP QLSEEIQCSL ASHIQSLVKS EKNRQVMCEA GLLGTLMASC HRALVTSGSP
   901  LHSRLIRIFE KLASQAIEPD VLRQFLGLGI PSSLSATTKI LDSSHTHRGN PGCSGSQTAQ
   961  GLAEGPWPAA PDAGLHPGVT QAPQPLGESQ DSTTALQTAL SLISMTSPRN LQPQRAALAP
  1021  SFVEFDMSVE GYGCLFIPTL STVMGTSTEY SVSGGIGTGA TRPFPPPGGL TFSCWFLISR
  1081  HGAATEGHPL RFLTLVRHLA RTEQPFVCFS VSLCPDDLSL VVSTEEKEFQ PLDVMEPEDD
  1141  SEPSAGCQLQ VRCGQLLACG QWHHLAVVVT KEMKRHCTVS TCLDGQVIGS AKMLYIQALP
  1201  GPFLSMDPSA FVDVYGYIAT PRVWKQKSSL IWRLGPTYLF EEAISMETLE VINKLGPRYC
  1261  GNFQAVHVQG EDLDSEATPF VAEERVSFGL HIASSSITSV ADIRNAYNEV DSRLIAKEMN
  1321  ISSRDNAMPV FLLRNCAGHL SGSLRTIGAV AVGQLGVRVF HSSPAASSLD FIGGPAILLG
  1381  LISLATDDHT MYAAVKVLHS VLTSNAMCDF LMQHICGYQI MAFLLRKKAS LLNHRIFQLI
  1441  LSVAGTVELG FRSSAITNTG VFQHILCNFE LWMNTADNLE LSLFSHLLEI LQSPREGPRN
  1501  AEAAHQAQLI PKLIFLFNEP SLIPSKISTI IGILACQLRG HFSTQDLLRI GLFVVYTLKP
  1561  SSVNERQICM DGALDPSLPA GSQTSGKTIW LRNQLLEMLL SVISSPQLHL SSESKEEMFL
  1621  KLGPDWFLLL LQGHLHASTT VLALKLLLYF LASPSLRTRF RDGLCAGSWV ERSTEGVDIV
  1681  MDNLKSQSPL PEQSPCLLPG FRVLNDFLAH HVHIPEVYLI VSTFFLQTPL TELMDGPKDS
  1741  LDAMLQWLLQ RHHQEEVLQA GLCTEGALLL LEMLKATMSQ PLAGSEDGAW AQTFPASVLQ
  1801  FLSLVHRTYP QDPAWRAPEF LQTLAIAAFP LGAQKGVGAE STRNTSSPEA AAEGDSTVEG
  1861  LQAPTKAHPA RRKLREFTQL LLRELLLGAS SPKQWLPLEV LLEASPDHAT SQQKRDFQSE
  1921  VLLSAMELFH MTSGGDAAMF RDGKEPQPSA EAAAAPSLAN ISCFTQKLVE KLYSGMFSAD
  1981  PRHILLFILE HIMVVIETAS SQRDTVLSTL YSSLNKVILY CLSKPQQSLS ECLGLLSILG
  2041  FLQEHWDVVF ATYNSNISFL LCLMHCLLLL NERSYPEGFG LEPKPRMSTY HQVFLSPNED
  2101  VKEKREDLPS LSDVQHNIQK TVQTLWQQLV AQRQQTLEDA FKIDLSVKPG EREVKIEEVT
  2161  PLWEETMLKA WQHYLASEKK SLASRSNVAH HSKVTLWSGS LSSAMKLMPG RQAKDPECKT
  2221  EDFVSCIENY RRRGQELYAS LYKDHVQRRK CGNIKAANAW ARIQEQLFGE LGLWSQGEET
  2281  KPCSPWELDW REGPARMRKR IKRLSPLEAL SSGRHKESQD KNDHISQTNA ENQDELTLRE
  2341  AEGEPDEVGV DCTQLTFFPA LHESLHSEDF LELCRERQVI LQELLDKEKV TQKFSLVIVQ
  2401  GHLVSEGVLL FGHQHFYICE NFTLSPTGDV YCTRHCLSNI SDPFIFNLCS KDRSTDHYSC
  2461  QCHSYADMRE LRQARFLLQD IALEIFFHNG YSKFLVFYNN DRSKAFKSFC SFQPSLKGKA
  2521  TSEDTLSLRR YPGSDRIMLQ KWQKRDISNF EYLMYLNTAA GRTCNDYMQY PVFPWVLADY
  2581  TSETLNLANP KIFRDLSKPM GAQTKERKLK FIQRFKEVEK TEGDMTVQCH YYTHYSSAII
  2641  VASYLVRMPP FTQAFCALQG GSFDVADRMF HSVKSTWESA SRENMSDVRE LTPEFFYLPE
  2701  FLTNCNGVEF GCMQDGTVLG DVQLPPWADG DPRKFISLHR KALESDFVSA NLHHWIDLIF
  2761  GYKQQGPAAV DAVNIFHPYF YGDRMDLSSI TDPLIKSTIL GFVSNFGQVP KQLFTKPHPA
  2821  RTAAGKPLPG KDVSTPVSLP GHPQPFFYSL QSLRPSQVTV KDMYLFSLGS ESPKGAIGHI
  2881  VSTEKTILAV ERNKVLLPPL WNRTFSWGFD DFSCCLGSYG SDKVLMTFEN LAAWGRCLCA
  2941  VCPSPTTIVT SGTSTVVCVW ELSMTKGRPR GLRLRQALYG HTQAVTCLAA SVTFSLLVSG
  3001  SQDCTCILWD LDHLTHVTRL PAHREGISAI TISDVSGTIV SCAGAHLSLW NVNGQPLASI
  3061  TTAWGPEGAI TCCCLMEGPA WDTSQIIITG SQDGMVRVWK TEDVKMSVPG RPAGEEPPAQ
  3121  PPSPRGHKWE KNLALSRELD VSIALTGKPS KTSPAVTALA VSRNHTKLLV GDERGRIFCW
  3181  SADG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WDFY4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

27 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.3

gnomAD pLI

0.34

gnomAD missense Z

3.12

DepMap mean gene effect

0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• antigen processing and presentation
• autophagy
• CD8-positive, alpha-beta T cell activation
• cellular response to virus

Cellular components

• early endosome
• endoplasmic reticulum

Protein domainsUniProt · Pfam · InterPro

• BEACH domain
• WD40 repeat
• Armadillo-like helical
• PH-like domain superfamily
• WD40/YVTN repeat-like-containing domain superfamily
• Armadillo-type fold
• WD40 repeat, conserved site
• PH-BEACH domain
• WD40-repeat-containing domain superfamily
• BEACH domain superfamily
• BEACH domain-containing protein
• WD domain, G-beta repeat
• Beige/BEACH domain

KeywordsUniProt

• Autophagy
• Endoplasmic reticulum
• Endosome
• Repeat
• WD repeat

InteractionsUniProt · HPA

Protein binding partners of WDFY4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HSP90AB1