TYW1B

Protein identityUniProt · HPA

UniProt accession

Q6NUM6

Gene

TYW1B

Ensembl

ENSG00000277149

Chromosome

7

Canonical length

668 aa

Protein class

Enzymes, Predicted membrane proteins

Subcellular location

Nucleoplasm,Nucleoli,Nucleoli rim

OverviewNCBI Gene

Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014]

Canonical amino-acid sequenceUniProt

668 residues, UniProt reviewed canonical sequence.

>Q6NUM6|TYW1B
     1  MDPSADTWDL SSPLISLWIN RFYIYLGFAV SISLWICVQI VIEMQGFATV LAEAVTSLDL
    61  PVAIINLKEY DPDDHLIEEV TSKNVCVFLV ATYTDGLPTE SAEWFCKWLE EASIDFRFGK
   121  TYLKGMRDAV FGLGNSAYAS HFNKVGKNVD KWLWMLGVHR VMSRGEGDCD VVKSKHGSIE
   181  ANFRAWKTKF ISQLQALQKG ERKKSCGGHC KKGKCESHQH GSEEREEGSQ EQDELHHRDT
   241  KEEEPFESSS EEEFGGEDHQ SLNSIVDVED LGKIMDHVKK EKREKEQQEE KSGLFRNMGR
   301  NEDGERRAMI TPALREALTK QVDAPRERSL LQTHILWNES HRCMETTPSL ACANKCVFCW
   361  WHHNNPVGTE WLWKMDQPEM ILKEAIENHQ NMIKQFKGVP GVKAERFEEG MTVKHCALSL
   421  VGEPIMYPEI NRFLKLLHQC KISSFLVTNA QFPAEIRNLE PVTQLYVSVD ASTKDSLKKI
   481  DRPLFKDFWQ QFLDSLKALA VKQQRTVYRL MLVKAWNVDE LQAYAQLVSL GNPDFIEVKG
   541  VTYCRESSAS SLTMAHVPWH EEVVQFVREL VDLIPEYEIA CEHEHSNCLL IAHRKFKIGG
   601  EWWTWIDYNR FQELIQEYED SGGSKTFSAK DYMARTPHWA LFGANERSFD PKDTRHQRKN
   661  KSKAISGC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TYW1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Unknown

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

12 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.77

gnomAD pLI

0

gnomAD missense Z

-0.39

DepMap mean gene effect

-0.18

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• wybutosine biosynthetic process

Molecular functions

• 4 iron, 4 sulfur cluster binding
• FMN binding
• metal ion binding
• tRNA-4-demethylwyosine synthase activity

Protein domainsUniProt · Pfam · InterPro

• Flavodoxin-like
• Radical SAM
• Flavodoxin/nitric oxide synthase
• Aldolase-type TIM barrel
• tRNA wybutosine-synthesis
• Flavoprotein-like superfamily
• S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
• Radical SAM superfamily
• Flavodoxin
• Radical SAM superfamily
• Wyosine base formation

KeywordsUniProt

• 4Fe-4S
• Iron
• Iron-sulfur
• Lyase
• Metal-binding
• Nucleotide-binding
• S-adenosyl-L-methionine
• tRNA processing