Seroatlas · Human Serome Atlas

TYW1

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1

Also known as: FLJ10900, MGC23001, MGC60291, RSAFD1, TYW1_HUMAN, TYW1A, YPL207W

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NV66
Gene: TYW1
Ensembl: ENSG00000198874
Chromosome: 7
Canonical length: 732 aa
Protein class: Enzymes, Predicted membrane proteins
Subcellular location: Nucleoplasm,Nucleoli,Nucleoli rim

OverviewNCBI Gene

Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Canonical amino-acid sequenceUniProt

732 residues, UniProt reviewed canonical sequence.

>Q9NV66|TYW1
     1  MDPSADTWDL FSPLISLWIN RFYIYLGFAV SISLWICVQI VIKTQGKNLQ EKSVPKAAQD
    61  LMTNGYVSLQ EKDIFVSGVK IFYGSQTGTA KGFATVLAEA VTSLDLPVAI INLKEYDPDD
   121  HLIEEVTSKN VCVFLVATYT DGLPTESAEW FCKWLEEASI DFRFGKTYLK GMRYAVFGLG
   181  NSAYASHFNK VGKNVDKWLW MLGAHRVMSR GEGDCDVVKS KHGSIEADFR AWKTKFISQL
   241  QALQKGERKK SCGGHCKKGK CESHQHGSEE REEGSHEQDE LHHRDTEEEE PFESSSEEEF
   301  GGEDHQSLNS IVDVEDLGKI MDHVKKEKRE KEQQEEKSGL FRNMGRNEDG ERRAMITPAL
   361  REALTKQGYQ LIGSHSGVKL CRWTKSMLRG RGGCYKHTFY GIESHRCMET TPSLACANKC
   421  VFCWRHHTNP VGTEWRWKMD QPEMILKEAI ENHQNMIKQF KGVPGVKAER FEEGMTVKHC
   481  ALSLVGEPIM YPEINRFLKL LHQCKISSFL VTNAQFPAEI RNLEPVTQLY VSVDASTKDS
   541  LKKIDRPLFK DFWQRFLDSL KALAVKQQRT VYRLTLVKAW NVDELQAYAQ LVSLGNPDFI
   601  EVKGVTYCGE SSASSLTMAH VPWHEEVVQF VHELVDLIPE YEIACEHEHS NCLLIAHRKF
   661  KIGGEWWTWI DYNRFQELIQ EYEDSGGSKT FSAKDYMART PHWALFGASE RGFDPKDTRH
   721  QRKNKSKAIS GC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TYW1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 15 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 15 nTPM
choroid plexus: 14 nTPM
parathyroid gland: 12 nTPM
thyroid gland: 12 nTPM
liver: 11 nTPM
cervix: 11 nTPM

Single-cell type

cardiomyocytes: 350 nCPM
myonuclei: 258 nCPM
choroid plexus epithelial cells: 255 nCPM
epicardial cells: 250 nCPM
adipocytes: 178 nCPM
cone photoreceptor cells: 153 nCPM

Immune cell

naive CD8 T-cell: 12 nTPM
MAIT T-cell: 11 nTPM
NK-cell: 11 nTPM
naive CD4 T-cell: 11 nTPM
non-classical monocyte: 10 nTPM
naive B-cell: 9.9 nTPM

Brain region

choroid plexus: 17 nTPM
white matter: 13 nTPM
medulla oblongata: 12 nTPM
cerebellum: 12 nTPM
hypothalamus: 12 nTPM
midbrain: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.75
gnomAD pLI: 0
gnomAD missense Z: 0.98
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

wybutosine biosynthetic process

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TYW1 as an antibody target. Whether an autoantibody or antibody against TYW1 could matter depends on whether native TYW1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TYW1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TYW1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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