Seroatlas · Human Serome Atlas

TSSK3

Testis-specific serine/threonine-protein kinase 3

Also known as: SPOGA3, STK22C, TSSK3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96PN8
Gene: TSSK3
Ensembl: ENSG00000162526
Chromosome: 1
Canonical length: 268 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Nuclear bodies

OverviewNCBI Gene

This gene encodes a kinase expressed exclusively in the testis that is thought to play a role in either germ cell differentiation or mature sperm function. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

268 residues, UniProt reviewed canonical sequence.

>Q96PN8|TSSK3
     1  MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKV IDKMGGPEEF IQRFLPRELQ
    61  IVRTLDHKNI IQVYEMLESA DGKICLVMEL AEGGDVFDCV LNGGPLPESR AKALFRQMVE
   121  AIRYCHGCGV AHRDLKCENA LLQGFNLKLT DFGFAKVLPK SHRELSQTFC GSTAYAAPEV
   181  LQGIPHDSKK GDVWSMGVVL YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLSISADCQD
   241  LLKRLLEPDM ILRPSIEEVS WHPWLAST

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSSK3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

testis: 42 nTPM
bone marrow: 12 nTPM
cerebellum: 6.5 nTPM
skin: 4.4 nTPM
retina: 4 nTPM
spleen: 4 nTPM

Single-cell type

late spermatids: 2,410 nCPM
early spermatids: 370 nCPM
late primary spermatocytes: 99 nCPM
sertoli cells: 68 nCPM
epicardial cells: 59 nCPM
cardiomyocytes: 28 nCPM

Immune cell

naive CD4 T-cell: 0.4 nTPM
gdT-cell: 0.2 nTPM
intermediate monocyte: 0.1 nTPM
naive B-cell: 0.1 nTPM
naive CD8 T-cell: 0.1 nTPM
non-classical monocyte: 0.1 nTPM

Brain region

cerebellum: 7.8 nTPM
white matter: 4.2 nTPM
medulla oblongata: 4 nTPM
choroid plexus: 3.9 nTPM
cerebral cortex: 3.8 nTPM
thalamus: 3.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.04
gnomAD pLI: 0.06
gnomAD missense Z: 0.01
DepMap mean gene effect: -0.22
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

sperm flagellum

Protein domainsUniProt · Pfam · InterPro

Protein kinase domain
Protein kinase-like domain superfamily
Protein kinase, ATP binding site
Protein kinase domain
Testis-specific serine/threonine-protein kinase 3, catalytic domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSSK3 as an antibody target. Whether an autoantibody or antibody against TSSK3 could matter depends on whether native TSSK3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSSK3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSSK3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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