Seroatlas · Human Serome Atlas

TSPY3

Testis-specific Y-encoded protein 3

Also known as: CT78, TSPY3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P0CV98
Gene
TSPY3
Ensembl
ENSG00000228927
Chromosome
Y
Canonical length
308 aa
Protein class
Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Predicted to enable chromatin binding activity and histone binding activity. Predicted to be involved in sex differentiation. Predicted to be located in cytoplasm. Predicted to be active in chromatin and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

308 residues, UniProt reviewed canonical sequence.

>P0CV98|TSPY3
     1  MRPEGSLTYR VPERLRQGFC GVGRAAQALV CASAKEGTAF RMEAVQEGAA GVESEQAALG
    61  EEAVLLLDDI MAEVEVVAEE EGLVERREEA QRAQQAVPGP GPMTPESALE ELLAVQVELE
   121  PVNAQARKAF SRQREKMERR RKPHLDRRGA VIQSVPGFWA NVIANHPQMS ALITDEDEDM
   181  LSYMVSLEVE EEKHPVHLCK IMLFFRSNPY FQNKVITKEY LVNITEYRAS HSTPIEWYPD
   241  YEVEAYRRRH HNSSLNFFNW FSDHNFAGSN KIAEILCKDL WRNPLQYYKR MKPPEEGTET
   301  SGDSQLLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPY3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
2.8 nTPM

Expression across tissuesHPA

Tissue

  • testis: 2.8 nTPM
  • epididymis: 0.5 nTPM
  • esophagus: 0.2 nTPM
  • adipose tissue: 0 nTPM
  • adrenal gland: 0 nTPM
  • amygdala: 0 nTPM

Single-cell type

  • differentiating spermatogonia: 6.6 nCPM
  • early primary spermatocytes: 2.9 nCPM
  • undifferentiated spermatogonia: 2.1 nCPM
  • early spermatids: 0.4 nCPM
  • adipocytes: 0 nCPM
  • adrenal cortex cells: 0 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • amygdala: 0 nTPM
  • basal ganglia: 0 nTPM
  • cerebellum: 0 nTPM
  • cerebral cortex: 0 nTPM
  • choroid plexus: 0 nTPM
  • hippocampal formation: 0 nTPM

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPY3 as an antibody target. Whether an autoantibody or antibody against TSPY3 could matter depends on whether native TSPY3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPY3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSPY3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TSPY3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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