Seroatlas · Human Serome Atlas

TSPY2

Testis-specific Y-encoded protein 2

Also known as: TSPY2_HUMAN, TSPYQ1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A6NKD2
Gene: TSPY2
Ensembl: ENSG00000168757
Chromosome: Y
Canonical length: 308 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Predicted to enable chromatin binding activity and histone binding activity. Predicted to be involved in several processes, including gonadal mesoderm development; nucleosome assembly; and spermatogenesis. Predicted to be located in cytoplasm. Predicted to be active in chromatin and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

308 residues, UniProt reviewed canonical sequence.

>A6NKD2|TSPY2
     1  MRPEGSLTYR VPERLRQGSC GVGRAAQALV CASAKEGTAF RMEAVQEGAA GVESEQAALG
    61  EEAVLLLDDI MAEVEVVAEE EGLVERREEA QRAQQAVPGP GPMTPESALE ELLAVQVELE
   121  PVNAQARKAF SRQREKMERR RKPHLDRRGA VIQSVPGFWA NVIANHPQMS ALITDEDEDM
   181  LSYMVSLEVE EEKHRVHLCK IMLFFRSNPY FQNKVITKEY LVNITEYRAS HSTPIEWYPD
   241  YEVEAYRRRH HNSSLNFFNW FSDHNFAGSN KIAEILCKDL WRNPLQYYKR MKPPEEGTET
   301  SGDSQLLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPY2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 7.2 nTPM

Expression across tissuesHPA

Tissue

testis: 7.2 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM

Single-cell type

differentiating spermatogonia: 27 nCPM
undifferentiated spermatogonia: 20 nCPM
early primary spermatocytes: 14 nCPM
extravillous trophoblasts: 0.7 nCPM
late spermatids: 0.7 nCPM
early spermatids: 0.3 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 0.4 nTPM
cerebral cortex: 0.1 nTPM
spinal cord: 0.1 nTPM
thalamus: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.89
gnomAD pLI: 0.1
gnomAD missense Z: -0.59

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPY2 as an antibody target. Whether an autoantibody or antibody against TSPY2 could matter depends on whether native TSPY2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPY2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSPY2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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