Seroatlas · Human Serome Atlas

TSPAN32

Tetraspanin-32

Also known as: PHEMX, TSN32_HUMAN, TSSC6

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96QS1
Gene
TSPAN32
Ensembl
ENSG00000064201
Chromosome
11
Canonical length
320 aa
Protein class
Predicted membrane proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

320 residues, UniProt reviewed canonical sequence.

>Q96QS1|TSPAN32
     1  MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW
    61  AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE
   121  DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR
   181  EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR
   241  ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR
   301  ALQGRSRGGL SGCPERGLSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPAN32 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.42
Highest tissue expression
19 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 19 nTPM
  • heart muscle: 11 nTPM
  • lymph node: 5.2 nTPM
  • spleen: 3.3 nTPM
  • tonsil: 2.3 nTPM
  • lung: 1.9 nTPM

Single-cell type

  • platelets: 116 nCPM
  • megakaryocyte progenitors: 85 nCPM
  • megakaryocyte-erythroid progenitors: 59 nCPM
  • hematopoietic stem cells: 59 nCPM
  • nk-cells: 55 nCPM
  • megakaryocytes: 43 nCPM

Immune cell

  • NK-cell: 59 nTPM
  • non-classical monocyte: 46 nTPM
  • intermediate monocyte: 41 nTPM
  • basophil: 41 nTPM
  • myeloid DC: 32 nTPM
  • classical monocyte: 32 nTPM

Brain region

  • cerebellum: 3 nTPM
  • medulla oblongata: 2.9 nTPM
  • white matter: 2.9 nTPM
  • midbrain: 2.6 nTPM
  • pons: 2.6 nTPM
  • cerebral cortex: 2.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.99
gnomAD pLI
0
gnomAD missense Z
-0.33
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPAN32 as an antibody target. Whether an autoantibody or antibody against TSPAN32 could matter depends on whether native TSPAN32 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPAN32 is annotated at the cell surface, where native TSPAN32 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TSPAN32 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TSPAN32. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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