Seroatlas · Human Serome Atlas

TRUB1

Pseudouridylate synthase TRUB1

Also known as: PUS4, TRUB1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WWH5
Gene: TRUB1
Ensembl: ENSG00000165832
Chromosome: 10
Canonical length: 349 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

349 residues, UniProt reviewed canonical sequence.

>Q8WWH5|TRUB1
     1  MAASEAAVVS SPSLKTDTSP VLETAGTVAA MAATPSARAA AAVVAAAART GSEARVSKAA
    61  LATKLLSLSG VFAVHKPKGP TSAELLNRLK EKLLAEAGMP SPEWTKRKKQ TLKIGHGGTL
   121  DSAARGVLVV GIGSGTKMLT SMLSGSKRYT AIGELGKATD TLDSTGRVTE EKPYDKITQE
   181  DIEGILQKFT GNIMQVPPLY SALKKDGQRL STLMKRGEVV EAKPARPVTV YSISLQKFQP
   241  PFFTLDVECG GGFYIRSLVS DIGKELSSCA NVLELTRTKQ GPFTLEEHAL PEDKWTIDDI
   301  AQSLEHCSSL FPAELALKKS KPESNEQVLS CEYITLNEPK REDDVIKTC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRUB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

adrenal gland: 28 nTPM
heart muscle: 20 nTPM
testis: 17 nTPM
ovary: 16 nTPM
kidney: 15 nTPM
parathyroid gland: 15 nTPM

Single-cell type

adrenal cortex cells: 76 nCPM
late primary spermatocytes: 59 nCPM
early spermatids: 56 nCPM
erythrocyte progenitors: 44 nCPM
choroid plexus epithelial cells: 40 nCPM
parietal cells: 37 nCPM

Immune cell

NK-cell: 8.1 nTPM
non-classical monocyte: 7.4 nTPM
memory B-cell: 7.3 nTPM
MAIT T-cell: 6.5 nTPM
T-reg: 6.3 nTPM
naive CD4 T-cell: 6.2 nTPM

Brain region

cerebral cortex: 15 nTPM
choroid plexus: 14 nTPM
basal ganglia: 14 nTPM
hypothalamus: 14 nTPM
white matter: 12 nTPM
thalamus: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.13
gnomAD pLI: 0
gnomAD missense Z: 0.5
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 1% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Pseudouridine synthase II, N-terminal
Pseudouridine synthase, catalytic domain superfamily
TruB family pseudouridylate synthase (N terminal domain)
tRNA pseudouridine synthase II, TruB

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRUB1 as an antibody target. Whether an autoantibody or antibody against TRUB1 could matter depends on whether native TRUB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRUB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRUB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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