Seroatlas · Human Serome Atlas

TRMT2B

tRNA (uracil-5-)-methyltransferase homolog B

Also known as: CXorf34, FLJ12687, TRM2B_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96GJ1
Gene: TRMT2B
Ensembl: ENSG00000188917
Chromosome: X
Canonical length: 504 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]

Canonical amino-acid sequenceUniProt

504 residues, UniProt reviewed canonical sequence.

>Q96GJ1|TRMT2B
     1  MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC
    61  QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KVKFAAQKKI LQRLESYIQM
   121  LNGVSVTTAV PKSERLSCLL HPIIPSPVIN GYRNKSTFSV NRGPDGNPKT VGFYLGTWRD
   181  GNVVCVQSNH LKNIPEKHSQ VAQYYEVFLR QSPLEPCLVF HEGGYWRELT VRTNSQGHTM
   241  AIITFHPQKL SQEELHVQKE IVKEFFIRGP GAACGLTSLY FQESTMTRCS HQQSPYQLLF
   301  GEPYIFEELL SLKIRISPDA FFQINTAGAE MLYRTVGELT GVNSDTILLD ICCGTGVIGL
   361  SLAQHTSRVL GIELLEQAVE DARWTAAFNG ITNSEFHTGQ AEKILPGLLK SKEDGQSIVA
   421  VVNPARAGLH YKVIQAIRNF RAIHTLVFVS CKLHGESTRN VIELCCPPDP AKKLLGEPFV
   481  LQQAVPVDLF PHTPHCELVL LFTR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRMT2B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 14 nTPM
parathyroid gland: 14 nTPM
thymus: 14 nTPM
tongue: 14 nTPM
tonsil: 11 nTPM
spleen: 9.8 nTPM

Single-cell type

distal convoluted tubule cells: 45 nCPM
renal collecting duct intercalated cells: 45 nCPM
myonuclei: 43 nCPM
renal connecting tubule cells: 39 nCPM
loop of henle epithelial cells: 34 nCPM
podocytes: 33 nCPM

Immune cell

naive CD4 T-cell: 22 nTPM
naive B-cell: 20 nTPM
eosinophil: 19 nTPM
NK-cell: 19 nTPM
memory B-cell: 19 nTPM
memory CD4 T-cell: 19 nTPM

Brain region

choroid plexus: 16 nTPM
pons: 14 nTPM
thalamus: 13 nTPM
medulla oblongata: 13 nTPM
midbrain: 13 nTPM
cerebral cortex: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.02
gnomAD pLI: 0
gnomAD missense Z: 1.03
DepMap mean gene effect: 0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

tRNA processing

Molecular functions

tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent
rRNA (uridine-C5-)-methyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

(Uracil-5)-methyltransferase family
Methyltransferase domain
S-adenosyl-L-methionine-dependent methyltransferase superfamily
tRNA (uracil(54)-C(5))-methyltransferase, metazoa type
Methyltransferase domain
tRNA (uracil-5-)-methyltransferase homolog B, chordates

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRMT2B as an antibody target. Whether an autoantibody or antibody against TRMT2B could matter depends on whether native TRMT2B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRMT2B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRMT2B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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