Seroatlas · Human Serome Atlas

TRMT12

tRNA wybutosine-synthesizing protein 2 homolog

Also known as: FLJ20772, Trm12, TYW2, TYW2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q53H54
Gene
TRMT12
Ensembl
ENSG00000183665
Chromosome
8
Canonical length
448 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Mitochondria

OverviewNCBI Gene

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

448 residues, UniProt reviewed canonical sequence.

>Q53H54|TRMT12
     1  MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE KMPDGSVALP
    61  VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG CSPAQKLCLE VSRWVEGRGV
   121  KWSAELEADL PRSWQRHGNL LLLSEDCFQA KQWKNLGPEL WETVALALGV QRLAKRGRVS
   181  PDGTRTPAVT LLLGDHGWVE HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD
   241  LYAGIGYFTL PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS
   301  NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL GVSKVEKEHW
   361  LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE TRIATLLQQV HGKPWKTQIL
   421  HIQPVKSYAP HVDHIVLDLE CCPCPSVG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRMT12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
9.7 nTPM

Expression across tissuesHPA

Tissue

  • choroid plexus: 9.7 nTPM
  • tongue: 9.3 nTPM
  • skeletal muscle: 9.2 nTPM
  • breast: 8.2 nTPM
  • thyroid gland: 8.2 nTPM
  • parathyroid gland: 8 nTPM

Single-cell type

  • late spermatids: 36 nCPM
  • choroid plexus epithelial cells: 28 nCPM
  • late primary spermatocytes: 25 nCPM
  • retinal pigment epithelial cells: 17 nCPM
  • ependymal cells: 15 nCPM
  • melanocytes: 15 nCPM

Immune cell

  • naive CD4 T-cell: 4 nTPM
  • naive CD8 T-cell: 3.9 nTPM
  • eosinophil: 3.8 nTPM
  • classical monocyte: 3.5 nTPM
  • non-classical monocyte: 3.3 nTPM
  • memory CD4 T-cell: 3.2 nTPM

Brain region

  • cerebral cortex: 16 nTPM
  • hippocampal formation: 16 nTPM
  • choroid plexus: 14 nTPM
  • hypothalamus: 14 nTPM
  • basal ganglia: 13 nTPM
  • white matter: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.53
gnomAD pLI
0.33
gnomAD missense Z
0.15
DepMap mean gene effect
-0.15
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 19% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRMT12 as an antibody target. Whether an autoantibody or antibody against TRMT12 could matter depends on whether native TRMT12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRMT12 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRMT12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TRMT12. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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