TPI1

Protein identityUniProt · HPA

UniProt accession

P60174

Gene

TPI1

Ensembl

ENSG00000111669

Chromosome

12

Canonical length

249 aa

Protein class

Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Canonical amino-acid sequenceUniProt

249 residues, UniProt reviewed canonical sequence.

>P60174|TPI1
     1  MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI
    61  AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE
   121  GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ
   181  QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE
   241  FVDIINAKQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TPI1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

2,316 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TPI1.

Disease | AllUniProt

Conditions TPI1 is implicated in, by any mechanism.

• Triosephosphate isomerase deficiency (TPID) MIM:615512

Disease | GeneticClinVar

11 pathogenic / likely-pathogenic of 180 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Triosephosphate isomerase deficiency

Disease | AutoantibodyPubMed

Conditions in which antibodies against TPI1 are reported. Each links to that disease's full target list.

Showing 0 of 2 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for TPI1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.73

gnomAD pLI

0.03

gnomAD missense Z

-0.22

DepMap mean gene effect

-0.81

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• canonical glycolysis
• gluconeogenesis
• glyceraldehyde-3-phosphate biosynthetic process
• glycerol catabolic process
• glycolytic process
• methylglyoxal biosynthetic process

Molecular functions

• protein homodimerization activity
• ubiquitin protein ligase binding
• methylglyoxal synthase activity
• triose-phosphate isomerase activity

Cellular components

• cytosol
• extracellular exosome
• extracellular space
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Aldolase-type TIM barrel
• Triosephosphate isomerase
• Triosephosphate isomerase, active site
• Triosephosphate isomerase, bacterial/eukaryotic
• Triosephosphate isomerase superfamily
• Triosephosphate isomerase

KeywordsUniProt

• Acetylation
• Alternative promoter usage
• Cytoplasm
• Gluconeogenesis
• Glycolysis
• Hereditary hemolytic anemia
• Isomerase
• Isopeptide bond
• Lyase
• Methylation
• Nitration
• Phosphoprotein
• Ubl conjugation