Seroatlas · Human Serome Atlas

TNFSF8

Tumor necrosis factor ligand superfamily member 8

Also known as: CD153, CD30LG, TNFL8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P32971
Gene
TNFSF8
Ensembl
ENSG00000106952
Chromosome
9
Canonical length
234 aa
Protein class
Cancer-related genes, CD markers, Predicted membrane proteins
Quaternary structure
Homotrimer

OverviewNCBI Gene

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Canonical amino-acid sequenceUniProt

234 residues, UniProt reviewed canonical sequence.

>P32971|TNFSF8
     1  MDPGLQQALN GMAPPGDTAM HVPAGSVASH LGTTSRSYFY LTTATLALCL VFTVATIMVL
    61  VVQRTDSIPN SPDNVPLKGG NCSEDLLCIL KRAPFKKSWA YLQVAKHLNK TKLSWNKDGI
   121  LHGVRYQDGN LVIQFPGLYF IICQLQFLVQ CPNNSVDLKL ELLINKHIKK QALVTVCESG
   181  MQTKHVYQNL SQFLLDYLQV NTTISVNVDT FQYIDTSTFP LENVLSIFLY SNSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TNFSF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
25 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 25 nTPM
  • lymph node: 22 nTPM
  • tonsil: 21 nTPM
  • bone marrow: 18 nTPM
  • appendix: 13 nTPM
  • spleen: 7.4 nTPM

Single-cell type

  • t-cells: 169 nCPM
  • thymocytes: 86 nCPM
  • neutrophils: 79 nCPM
  • monocytes: 65 nCPM
  • innate lymphoid cells: 51 nCPM
  • b-cells: 45 nCPM

Immune cell

  • naive CD4 T-cell: 26 nTPM
  • memory CD4 T-cell: 24 nTPM
  • classical monocyte: 21 nTPM
  • MAIT T-cell: 20 nTPM
  • naive CD8 T-cell: 20 nTPM
  • memory CD8 T-cell: 14 nTPM

Brain region

  • thalamus: 3.3 nTPM
  • white matter: 2.8 nTPM
  • medulla oblongata: 2.1 nTPM
  • choroid plexus: 1.7 nTPM
  • spinal cord: 1.5 nTPM
  • pons: 1.3 nTPM

ReferencesPubMed · IEDB

Publications for TNFSF8 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

1 publication

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.53
gnomAD pLI
0.76
gnomAD missense Z
0.83
DepMap mean gene effect
0.06
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TNFSF8 as an antibody target. Whether an autoantibody or antibody against TNFSF8 could matter depends on whether native TNFSF8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TNFSF8 is annotated at the cell surface, where native TNFSF8 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TNFSF8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TNFSF8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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