Seroatlas · Human Serome Atlas

TNFSF4

Tumor necrosis factor ligand superfamily member 4

Also known as: CD252, gp34, OX-40L, TNFL4_HUMAN, TXGP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P23510
Gene
TNFSF4
Ensembl
ENSG00000117586
Chromosome
1
Canonical length
183 aa
Protein class
Cancer-related genes, CD markers, Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles
Quaternary structure
Homotrimer

OverviewNCBI Gene

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

183 residues, UniProt reviewed canonical sequence.

>P23510|TNFSF4
     1  MERVQPLEEN VGNAARPRFE RNKLLLVASV IQGLGLLLCF TYICLHFSAL QVSHRYPRIQ
    61  SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS QEVNISLHYQ
   121  KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL ILIHQNPGEF
   181  CVL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TNFSF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
3.7 nTPM

Expression across tissuesHPA

Tissue

  • testis: 3.7 nTPM
  • thymus: 3.5 nTPM
  • appendix: 2.1 nTPM
  • lymph node: 2.1 nTPM
  • adrenal gland: 1.9 nTPM
  • cerebellum: 1.9 nTPM

Single-cell type

  • platelets: 442 nCPM
  • thymocytes: 43 nCPM
  • innate lymphoid cells: 37 nCPM
  • hematopoietic stem cells: 28 nCPM
  • megakaryocyte progenitors: 9.6 nCPM
  • astrocytes: 8.9 nCPM

Immune cell

  • total PBMC: 2.8 nTPM
  • NK-cell: 1.7 nTPM
  • plasmacytoid DC: 1.2 nTPM
  • neutrophil: 0.7 nTPM
  • gdT-cell: 0.5 nTPM
  • memory B-cell: 0.5 nTPM

Brain region

  • cerebellum: 4.6 nTPM
  • basal ganglia: 4 nTPM
  • cerebral cortex: 4 nTPM
  • medulla oblongata: 3.9 nTPM
  • pons: 3.9 nTPM
  • midbrain: 3.7 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TNFSF4.

Disease | AllUniProt

Conditions TNFSF4 is implicated in, by any mechanism.

ReferencesPubMed · IEDB

Publications for TNFSF4 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

1 publication

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.68
gnomAD pLI
0.05
gnomAD missense Z
0.92
DepMap mean gene effect
0.09
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TNFSF4 as an antibody target. Whether an autoantibody or antibody against TNFSF4 could matter depends on whether native TNFSF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TNFSF4 is annotated at the cell surface, where native TNFSF4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TNFSF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TNFSF4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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