Seroatlas · Human Serome Atlas

TMEM74B

Transmembrane protein 74B

Also known as: C20orf46, FLJ11190, TM74B_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NUR3
Gene
TMEM74B
Ensembl
ENSG00000125895
Chromosome
20
Canonical length
256 aa
Protein class
Predicted membrane proteins
Subcellular location
Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

256 residues, UniProt reviewed canonical sequence.

>Q9NUR3|TMEM74B
     1  MPPAQGYEFA AAKGPRDELG PSFPMASPPG LELKTLSNGP QAPRRSAPLG PVAPTREGVE
    61  NACFSSEEHE THFQNPGNTR LGSSPSPPGG VSSLPRSQRD DLSLHSEEGP ALEPVSRPVD
   121  YGFVSALVFL VSGILLVVTA YAIPREARVN PDTVTAREME RLEMYYARLG SHLDRCIIAG
   181  LGLLTVGGML LSVLLMVSLC KGELYRRRTF VPGKGSRKTY GSINLRMRQL NGDGGQALVE
   241  NEVVQVSETS HTLQRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM74B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
2
Mean surface accessibility (rSASA)
0.63
Highest tissue expression
27 nTPM

Expression across tissuesHPA

Tissue

  • basal ganglia: 27 nTPM
  • lung: 23 nTPM
  • small intestine: 18 nTPM
  • cerebral cortex: 17 nTPM
  • hypothalamus: 15 nTPM
  • duodenum: 14 nTPM

Single-cell type

  • tuft cells: 46 nCPM
  • brain inhibitory neurons: 27 nCPM
  • retinal ganglion cells: 27 nCPM
  • fallopian tube ciliated cells: 23 nCPM
  • pericytes: 21 nCPM
  • goblet cells: 19 nCPM

Immune cell

  • memory B-cell: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • pons: 24 nTPM
  • hypothalamus: 21 nTPM
  • amygdala: 20 nTPM
  • basal ganglia: 18 nTPM
  • cerebral cortex: 18 nTPM
  • medulla oblongata: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.5
gnomAD pLI
0
gnomAD missense Z
-0.43
DepMap mean gene effect
0.06
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM74B as an antibody target. Whether an autoantibody or antibody against TMEM74B could matter depends on whether native TMEM74B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM74B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM74B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMEM74B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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