Seroatlas · Human Serome Atlas

TMEM74

Transmembrane protein 74

Also known as: FLJ30668, NET36, TMM74_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96NL1
Gene: TMEM74
Ensembl: ENSG00000164841
Chromosome: 8
Canonical length: 305 aa
Protein class: Predicted membrane proteins, Transporters
Subcellular location: Golgi apparatus,Cytosol

OverviewNCBI Gene

Predicted to enable transmembrane transporter binding activity. Involved in macroautophagy. Predicted to be located in cytoplasmic vesicle; plasma membrane; and vacuolar membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

305 residues, UniProt reviewed canonical sequence.

>Q96NL1|TMEM74
     1  MELHYLAKKS NQADLCDARD WSSRGLPGDQ ADTAATRAAL CCQKQCASTP RATEMEGSKL
    61  SSSPASPSSS LQNSTLQPDA FPPGLLHSGN NQITAERKVC NCCSQELETS FTYVDKNINL
   121  EQRNRSSPSA KGHNHPGELG WENPNEWSQE AAISLISEEE DDTSSEATSS GKSIDYGFIS
   181  AILFLVTGIL LVIISYIVPR EVTVDPNTVA AREMERLEKE SARLGAHLDR CVIAGLCLLT
   241  LGGVILSCLL MMSMWKGELY RRNRFASSKE SAKLYGSFNF RMKTSTNENT LELSLVEEDA
   301  LAVQS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM74 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 2.7 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 2.7 nTPM
retina: 2.3 nTPM
cerebellum: 2.2 nTPM
hippocampal formation: 2 nTPM
spinal cord: 2 nTPM
amygdala: 1.4 nTPM

Single-cell type

epididymal principal cells: 192 nCPM
bergmann glia: 64 nCPM
brain excitatory neurons: 15 nCPM
ependymal cells: 15 nCPM
granulosa cells: 9.7 nCPM
astrocytes: 9.4 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 17 nTPM
spinal cord: 13 nTPM
cerebellum: 9.6 nTPM
medulla oblongata: 8 nTPM
hippocampal formation: 7.9 nTPM
hypothalamus: 6.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.47
gnomAD pLI: 0
gnomAD missense Z: 0.05
DepMap mean gene effect: 0.1
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

transmembrane transporter binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM74 as an antibody target. Whether an autoantibody or antibody against TMEM74 could matter depends on whether native TMEM74 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM74 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM74 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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