TMEM65
Transmembrane protein 65
Also known as: TMM65_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q6PI78
- Gene
- TMEM65
- Ensembl
- ENSG00000164983
- Chromosome
- 8
- Canonical length
- 240 aa
- Protein class
- Disease related genes, Potential drug targets, Predicted membrane proteins, Transporters
- Subcellular location
- Vesicles,Plasma membrane
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Predicted to be involved in cardiac conduction; cardiac ventricle development; and regulation of cardiac conduction. Located in several cellular components, including intercalated disc; mitochondrial inner membrane; and nucleolus. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
240 residues, UniProt reviewed canonical sequence.
>Q6PI78|TMEM65
1 MSRLLPLLRS RTARSLRPGP AAAAAPRPPS WCCCGRGLLA LAPPGGLPGG PRRLGTHPKK
61 EPMEALNTAQ GARDFIYSLH STERSCLLKE LHRFESIAIA QEKLEAPPPT PGQLRYVFIH
121 NAIPFIGFGF LDNAIMIVAG THIEMSIGII LGISTMAAAA LGNLVSDLAG LGLAGYVEAL
181 ASRLGLSIPD LTPKQVDMWQ TRLSTHLGKA VGVTIGCILG MFPLIFFGGG EEDEKLETKSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TMEM65 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 3
- Mean surface accessibility (rSASA)
- 0.48
- Highest tissue expression
- 27 nTPM
Expression across tissuesHPA
Tissue
- duodenum: 27 nTPM
- heart muscle: 25 nTPM
- skeletal muscle: 25 nTPM
- tongue: 22 nTPM
- cerebral cortex: 18 nTPM
- salivary gland: 16 nTPM
Single-cell type
- myonuclei: 440 nCPM
- cardiomyocytes: 392 nCPM
- retinal horizontal cells: 343 nCPM
- neutrophils: 305 nCPM
- pituitary stem cells: 240 nCPM
- renal collecting duct intercalated cells: 211 nCPM
Immune cell
- basophil: 1 nTPM
- eosinophil: 0.9 nTPM
- NK-cell: 0.8 nTPM
- T-reg: 0.8 nTPM
- neutrophil: 0.7 nTPM
- memory B-cell: 0.6 nTPM
Brain region
- midbrain: 28 nTPM
- thalamus: 28 nTPM
- pons: 26 nTPM
- cerebellum: 26 nTPM
- hypothalamus: 26 nTPM
- cerebral cortex: 25 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.84
- gnomAD pLI
- 0.11
- gnomAD missense Z
- 1.38
- DepMap mean gene effect
- 0.03
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 14% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Transmembrane protein 65
- Transmembrane protein 65
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of TMEM65 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TMEM65 as an antibody target. Whether an autoantibody or antibody against TMEM65 could matter depends on whether native TMEM65 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TMEM65 is annotated at the cell surface, where native TMEM65 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label TMEM65 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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