Seroatlas · Human Serome Atlas

TMEM254

Transmembrane protein 254

Also known as: bA369J21.6, C10orf57, FLJ13263, TM254_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TBM7
Gene
TMEM254
Ensembl
ENSG00000133678
Chromosome
10
Canonical length
123 aa
Protein class
Predicted membrane proteins
Subcellular location
Nucleoplasm,Plasma membrane

OverviewNCBI Gene

Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

123 residues, UniProt reviewed canonical sequence.

>Q8TBM7|TMEM254
     1  MATAAGATYF QRGSLFWFTV ITLSFGYYTW VVFWPQSIPY QNLGPLGPFT QYLVDHHHTL
    61  LCNGYWLAWL IHVGESLYAI VLCKHKGITS GRAQLLWFLQ TFFFGIASLT ILIAYKRKRQ
   121  KQT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM254 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
3
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
68 nTPM

Expression across tissuesHPA

Tissue

  • skin: 68 nTPM
  • epididymis: 59 nTPM
  • liver: 49 nTPM
  • prostate: 42 nTPM
  • esophagus: 36 nTPM
  • fallopian tube: 26 nTPM

Single-cell type

  • esophageal apical cells: 167 nCPM
  • respiratory ciliated cells: 108 nCPM
  • ependymal cells: 94 nCPM
  • epididymal principal cells: 94 nCPM
  • esophageal suprabasal cells: 91 nCPM
  • fallopian tube ciliated cells: 80 nCPM

Immune cell

  • basophil: 58 nTPM
  • naive CD4 T-cell: 34 nTPM
  • non-classical monocyte: 32 nTPM
  • intermediate monocyte: 29 nTPM
  • T-reg: 27 nTPM
  • classical monocyte: 25 nTPM

Brain region

  • white matter: 17 nTPM
  • midbrain: 15 nTPM
  • medulla oblongata: 15 nTPM
  • spinal cord: 15 nTPM
  • basal ganglia: 15 nTPM
  • hippocampal formation: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.48
gnomAD pLI
0
gnomAD missense Z
-0.32
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Transmembrane protein 254
  • Transmembrane protein 254

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM254 as an antibody target. Whether an autoantibody or antibody against TMEM254 could matter depends on whether native TMEM254 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM254 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM254 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMEM254. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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