Seroatlas · Human Serome Atlas

TMEM253

Transmembrane protein 253

Also known as: C14orf176, C14orf95, NCRNA00220, TM253_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P0C7T8
Gene: TMEM253
Ensembl: ENSG00000232070
Chromosome: 14
Canonical length: 217 aa
Protein class: Predicted membrane proteins

OverviewNCBI Gene

Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

217 residues, UniProt reviewed canonical sequence.

>P0C7T8|TMEM253
     1  MEDRAGEQEQ ERHSLRLEKL QHWARHRQSG HLLVLAVSQL WLAVVVVPLA VSVACLNSDC
    61  HMATALPLGP GASGLLTGTV TLELRRAPRL WKVRAMMIFN TFNLILGFIV VVVEVMKTAL
   121  GPAPTASSQH AGLLVLELSA EAFTLGGVLV SVHALFLLSQ RKPGCCRSQS LHYQELQEGF
   181  SELEEVPGLE NGPTVASTGA NERVGQREQT RAALLPP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM253 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 4
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 91 nTPM

Expression across tissuesHPA

Tissue

duodenum: 91 nTPM
small intestine: 83 nTPM
colon: 14 nTPM
rectum: 12 nTPM
salivary gland: 6.7 nTPM
thyroid gland: 4.8 nTPM

Single-cell type

enterocytes: 185 nCPM
colonocytes: 44 nCPM
enteric transient amplifying cells: 33 nCPM
enteric stem cells: 11 nCPM
goblet cells: 8 nCPM
platelets: 5.6 nCPM

Immune cell

neutrophil: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

cerebellum: 0.8 nTPM
midbrain: 0.7 nTPM
pons: 0.6 nTPM
white matter: 0.6 nTPM
hypothalamus: 0.5 nTPM
medulla oblongata: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.67
gnomAD pLI: 0
gnomAD missense Z: 0.59
DepMap mean gene effect: 0.12
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

Transmembrane protein 253

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM253 as an antibody target. Whether an autoantibody or antibody against TMEM253 could matter depends on whether native TMEM253 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM253 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM253 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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