Seroatlas · Human Serome Atlas

TMEM249

Cation channel sperm-associated auxiliary subunit TMEM249

Also known as: C8orfK29, TM249_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q2WGJ8
Gene
TMEM249
Ensembl
ENSG00000261587
Chromosome
8
Canonical length
235 aa
Protein class
Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

Predicted to be located in sperm principal piece. Predicted to be part of CatSper complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

235 residues, UniProt reviewed canonical sequence.

>Q2WGJ8|TMEM249
     1  MPKGRAGSLP TTSIGWRFQL WFLGLTCPER HLARRLKNNS FYPFVQQEPN VFVLEYYLDT
    61  LWKGMLLFII SVVLVSFSSL REVQKQETWV FLVYGVGVGL WLVISSLPRR RLVLNHTRGV
   121  YHFSIQGRTV CQGPLHLVYV RLALSSDAHG RCFFHLVLGG HRLEPLVLVQ LSEHYEQMEY
   181  LGRYIARKLN INYFDYLATS YRHVVRHWPP PGAGTVMGKS PMGHKPSSSQ SSLEV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM249 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
2
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
26 nTPM

Expression across tissuesHPA

Tissue

  • testis: 26 nTPM
  • cerebellum: 1.4 nTPM
  • amygdala: 0.6 nTPM
  • cerebral cortex: 0.6 nTPM
  • basal ganglia: 0.5 nTPM
  • hypothalamus: 0.5 nTPM

Single-cell type

  • late spermatids: 6.9 nCPM
  • brain excitatory neurons: 3.7 nCPM
  • astrocytes: 3.3 nCPM
  • late primary spermatocytes: 3.2 nCPM
  • brain inhibitory neurons: 3.1 nCPM
  • early spermatids: 3 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebellum: 1 nTPM
  • cerebral cortex: 1 nTPM
  • hypothalamus: 0.6 nTPM
  • pons: 0.6 nTPM
  • amygdala: 0.5 nTPM
  • thalamus: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.85
gnomAD pLI
0.1
gnomAD missense Z
0.89
DepMap mean gene effect
-0.09
DepMap dependency class
selective

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Cation channel sperm-associated auxiliary subunit TMEM249
  • Cation channel sperm-associated auxiliary subunit TMEM249

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM249 as an antibody target. Whether an autoantibody or antibody against TMEM249 could matter depends on whether native TMEM249 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM249 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM249 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMEM249. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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