Seroatlas · Human Serome Atlas

TMEM221

Transmembrane protein 221

Also known as: TM221_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
A6NGB7
Gene
TMEM221
Ensembl
ENSG00000188051
Chromosome
19
Canonical length
291 aa
Protein class
Predicted membrane proteins
Subcellular location
Nucleoplasm,Nuclear membrane,Cytosol

OverviewNCBI Gene

Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

291 residues, UniProt reviewed canonical sequence.

>A6NGB7|TMEM221
     1  MARSYGGRVL AAMTLLGIAA AVLAALGAQL LFQLQAGRAE LRGLRAEGLG QELGAGPGLP
    61  EDAAGTLLPL AAALAALVLV LGFTCLLLAA LCGHLGAELA RGPGPRRSDW FLYDCRLLRH
   121  VALGLFCCGI SVYLAALSIY ALLLFEIETG AAAASILGSG TLVLVAVLTH TLLRAARAAR
   181  RGLHELSPPS FEDDLARPAE VSKASPRAQP QQGIHRRTPY STCPEPGDPF GSMATATAPA
   241  ALEGGWESSL PASRMHRTLS AGLGHWDGVT HEMRRMLGHR PGSMGKDSTL V

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM221 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.5
Highest tissue expression
8.8 nTPM

Expression across tissuesHPA

Tissue

  • retina: 8.8 nTPM
  • ovary: 7.3 nTPM
  • parathyroid gland: 5.1 nTPM
  • cerebral cortex: 5 nTPM
  • adrenal gland: 4 nTPM
  • amygdala: 3.7 nTPM

Single-cell type

  • müller glia: 47 nCPM
  • medullary thymic epithelial cells: 34 nCPM
  • fallopian secretory cells: 9.1 nCPM
  • hofbauer cells: 8 nCPM
  • rod photoreceptor cells: 6.7 nCPM
  • thymic myoid cells: 4.1 nCPM

Immune cell

  • plasmacytoid DC: 0.2 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • hypothalamus: 3.9 nTPM
  • cerebral cortex: 3.8 nTPM
  • medulla oblongata: 3.5 nTPM
  • basal ganglia: 3.4 nTPM
  • amygdala: 3.3 nTPM
  • spinal cord: 3.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.85
gnomAD pLI
0.01
gnomAD missense Z
0.8
DepMap mean gene effect
0.02
DepMap dependency class
selective

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Jiraiya
  • Transmembrane protein 221
  • Jiraiya

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM221 as an antibody target. Whether an autoantibody or antibody against TMEM221 could matter depends on whether native TMEM221 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM221 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM221 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMEM221. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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