Seroatlas · Human Serome Atlas

TMEM169

Transmembrane protein 169

Also known as: FLJ34263, TM169_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96HH4
Gene: TMEM169
Ensembl: ENSG00000163449
Chromosome: 2
Canonical length: 297 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Focal adhesion sites

OverviewNCBI Gene

Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

297 residues, UniProt reviewed canonical sequence.

>Q96HH4|TMEM169
     1  MEEPTAVEGQ VQLPSPHQGS LRKAVAAALA LDGESTMGHR KKKRKESRPE SIIIYRSDNE
    61  KTDEEPGESE GGDQPKEEEG DDFLDYPVDD DMWNLPLDSR YVTLTGTITR GKKKGQMVDI
   121  HVTLTEKELQ ELTKPKESSR ETTPEGRMAC QMGADRGPHV VLWTLICLPV VFILSFVVSF
   181  YYGTITWYNI FLVYNEERTF WHKISYCPCL VLFYPVLIMA MASSLGLYAA VVQLSWSWEA
   241  WWQAARDMEK GFCGWLCSKL GLEDCSPYSI VELLESDNIS STLSNKDPIQ EVETSTV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM169 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 5.9 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 5.9 nTPM
cerebellum: 3.5 nTPM
retina: 3.2 nTPM
spinal cord: 2.7 nTPM
thyroid gland: 2.4 nTPM
hypothalamus: 2.3 nTPM

Single-cell type

cone photoreceptor cells: 33 nCPM
renal collecting duct principal cells: 25 nCPM
oligodendrocyte progenitor cells: 24 nCPM
retinal bipolar cells: 23 nCPM
retinal ganglion cells: 19 nCPM
myosatellite cells: 17 nCPM

Immune cell

T-reg: 3.7 nTPM
non-classical monocyte: 3.3 nTPM
neutrophil: 2.3 nTPM
naive CD4 T-cell: 2.1 nTPM
memory CD4 T-cell: 2 nTPM
memory B-cell: 1.5 nTPM

Brain region

cerebral cortex: 11 nTPM
pons: 10 nTPM
white matter: 10 nTPM
hypothalamus: 9 nTPM
hippocampal formation: 8.9 nTPM
midbrain: 8.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.89
gnomAD pLI: 0.01
gnomAD missense Z: 0.3
DepMap mean gene effect: 0
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

TMEM169 protein
TMEM169 protein family

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM169 as an antibody target. Whether an autoantibody or antibody against TMEM169 could matter depends on whether native TMEM169 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM169 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM169 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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