Seroatlas · Human Serome Atlas

TMEM116

Transmembrane protein 116

Also known as: FLJ90167, TM116_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NCL8
Gene: TMEM116
Ensembl: ENSG00000198270
Chromosome: 12
Canonical length: 245 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Microtubules

OverviewNCBI Gene

Predicted to be involved in signal transduction. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

245 residues, UniProt reviewed canonical sequence.

>Q8NCL8|TMEM116
     1  MKHTQSGQST SPLVIDYTCR VCQMAFVFSS LIPLLLMTPV FCLGNTSECF QNFSQSHKCI
    61  LMHSPPSAMA ELPPSANTSV CSTLYFYGIA IFLGSFVLSL LTIMVLLIRA QTLYKKFVKS
   121  TGFLGSEQWA VIHIVDQRVR FYPVAFFCCW GPAVILMIIK LTKPQDTKLH MALYVLQALT
   181  ATSQGLLNCG VYGWTQHKFH QLKQEARRDA DTQTPLLCSQ KRFYSRGLNS LESTLTFPAS
   241  TSTIF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM116 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 56 nTPM

Expression across tissuesHPA

Tissue

choroid plexus: 56 nTPM
parathyroid gland: 56 nTPM
kidney: 29 nTPM
testis: 25 nTPM
heart muscle: 25 nTPM
salivary gland: 13 nTPM

Single-cell type

renal collecting duct intercalated cells: 302 nCPM
choroid plexus epithelial cells: 264 nCPM
cardiomyocytes: 241 nCPM
early primary spermatocytes: 146 nCPM
late primary spermatocytes: 145 nCPM
distal convoluted tubule cells: 132 nCPM

Immune cell

T-reg: 19 nTPM
memory CD4 T-cell: 19 nTPM
memory CD8 T-cell: 16 nTPM
naive CD8 T-cell: 11 nTPM
naive CD4 T-cell: 9.7 nTPM
gdT-cell: 8.2 nTPM

Brain region

choroid plexus: 54 nTPM
cerebellum: 15 nTPM
white matter: 13 nTPM
basal ganglia: 12 nTPM
thalamus: 11 nTPM
midbrain: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.45
gnomAD pLI: 0
gnomAD missense Z: 0.85
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

signal transduction

Cellular components

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM116 as an antibody target. Whether an autoantibody or antibody against TMEM116 could matter depends on whether native TMEM116 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM116 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM116 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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