Seroatlas · Human Serome Atlas

TM4SF20

Transmembrane 4 L6 family member 20

Also known as: FLJ22800, T4S20_HUMAN, TCCE518

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q53R12
Gene
TM4SF20
Ensembl
ENSG00000168955
Chromosome
2
Canonical length
229 aa
Protein class
Disease related genes, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location
Plasma membrane,Focal adhesion sites

OverviewNCBI Gene

The protein encoded by this gene is a member of the four-transmembrane L6 superfamily. Members of this family function in various cellular processes including cell proliferation, motility, and adhesion via their interactions with integrins. In human brain tissue, this gene is expressed at high levels in the parietal lobe, occipital lobe, hippocampus, pons, white matter, corpus callosum, and cerebellum. Knockout of the homologous gene in mouse results in a neurobehavioral phenotype with suggested enhanced motor coordination. A deletion mutation in the human gene is associated with specific language impairment-5. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

229 residues, UniProt reviewed canonical sequence.

>Q53R12|TM4SF20
     1  MTCCEGWTSC NGFSLLVLLL LGVVLNAIPL IVSLVEEDQF SQNPISCFEW WFPGIIGAGL
    61  MAIPATTMSL TARKRACCNN RTGMFLSSLF SVITVIGALY CMLISIQALL KGPLMCNSPS
   121  NSNANCEFSL KNISDIHPES FNLQWFFNDS CAPPTGFNKP TSNDTMASGW RASSFHFDSE
   181  ENKHRLIHFS VFLGLLLVGI LEVLFGLSQI VIGFLGCLCG VSKRRSQIV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TM4SF20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
251 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 251 nTPM
  • small intestine: 136 nTPM
  • rectum: 3.6 nTPM
  • testis: 3.2 nTPM
  • colon: 2.9 nTPM
  • smooth muscle: 1.4 nTPM

Single-cell type

  • enterocytes: 796 nCPM
  • gastric progenitor cells: 272 nCPM
  • late spermatids: 117 nCPM
  • paneth cells: 43 nCPM
  • early spermatids: 37 nCPM
  • late primary spermatocytes: 34 nCPM

Immune cell

  • basophil: 0.1 nTPM
  • naive B-cell: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • NK-cell: 0.1 nTPM
  • plasmacytoid DC: 0.1 nTPM
  • classical monocyte: 0 nTPM

Brain region

  • cerebellum: 4.4 nTPM
  • white matter: 3.3 nTPM
  • cerebral cortex: 2.7 nTPM
  • choroid plexus: 2.7 nTPM
  • basal ganglia: 2.4 nTPM
  • medulla oblongata: 2.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TM4SF20.

Disease | AllUniProt

Conditions TM4SF20 is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.63
gnomAD pLI
0
gnomAD missense Z
-0.4
DepMap mean gene effect
0.1
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TM4SF20 as an antibody target. Whether an autoantibody or antibody against TM4SF20 could matter depends on whether native TM4SF20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TM4SF20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TM4SF20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TM4SF20. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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