Seroatlas · Human Serome Atlas

TKFC

Triokinase/FMN cyclase

Also known as: DAK, DKFZP586B1621, NET45, TKFC_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q3LXA3
Gene
TKFC
Ensembl
ENSG00000149476
Chromosome
11
Canonical length
575 aa
Protein class
Disease related genes, Enzymes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017]

Canonical amino-acid sequenceUniProt

575 residues, UniProt reviewed canonical sequence.

>Q3LXA3|TKFC
     1  MTSKKLVNSV AGCADDALAG LVACNPNLQL LQGHRVALRS DLDSLKGRVA LLSGGGSGHE
    61  PAHAGFIGKG MLTGVIAGAV FTSPAVGSIL AAIRAVAQAG TVGTLLIVKN YTGDRLNFGL
   121  AREQARAEGI PVEMVVIGDD SAFTVLKKAG RRGLCGTVLI HKVAGALAEA GVGLEEIAKQ
   181  VNVVAKAMGT LGVSLSSCSV PGSKPTFELS ADEVELGLGI HGEAGVRRIK MATADEIVKL
   241  MLDHMTNTTN ASHVPVQPGS SVVMMVNNLG GLSFLELGII ADATVRSLEG RGVKIARALV
   301  GTFMSALEMP GISLTLLLVD EPLLKLIDAE TTAAAWPNVA AVSITGRKRS RVAPAEPQEA
   361  PDSTAAGGSA SKRMALVLER VCSTLLGLEE HLNALDRAAG DGDCGTTHSR AARAIQEWLK
   421  EGPPPASPAQ LLSKLSVLLL EKMGGSSGAL YGLFLTAAAQ PLKAKTSLPA WSAAMDAGLE
   481  AMQKYGKAAP GDRTMLDSLW AAGQELQAWK SPGADLLQVL TKAVKSAEAA AEATKNMEAG
   541  AGRASYISSA RLEQPDPGAV AAAAILRAIL EVLQS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TKFC can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
181 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 181 nTPM
  • small intestine: 130 nTPM
  • liver: 108 nTPM
  • adrenal gland: 77 nTPM
  • kidney: 43 nTPM
  • testis: 27 nTPM

Single-cell type

  • enterocytes: 375 nCPM
  • hepatocytes: 154 nCPM
  • enteric transient amplifying cells: 72 nCPM
  • adrenal cortex cells: 65 nCPM
  • paneth cells: 54 nCPM
  • late primary spermatocytes: 50 nCPM

Immune cell

  • myeloid DC: 21 nTPM
  • classical monocyte: 13 nTPM
  • T-reg: 13 nTPM
  • memory B-cell: 10 nTPM
  • plasmacytoid DC: 10 nTPM
  • naive B-cell: 9.7 nTPM

Brain region

  • white matter: 15 nTPM
  • medulla oblongata: 14 nTPM
  • spinal cord: 14 nTPM
  • midbrain: 13 nTPM
  • cerebral cortex: 13 nTPM
  • pons: 13 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TKFC.

Disease | AllUniProt

Conditions TKFC is implicated in, by any mechanism.

  • Triokinase and FMN cyclase deficiency syndrome (TKFCD) MIM:618805

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 139 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Triokinase and FMN cyclase deficiency syndrome
  • TKFC deficiency
  • Inborn errors of metabolism

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.13
gnomAD pLI
0
DepMap mean gene effect
-0.18
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • DhaK domain
  • DhaL domain
  • Dihydroxyacetone kinase
  • DhaL domain superfamily
  • Dihydroxyacetone Kinase (DAK)
  • Dak1 domain
  • DAK2 domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TKFC in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TKFC as an antibody target. Whether an autoantibody or antibody against TKFC could matter depends on whether native TKFC is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TKFC is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TKFC as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TKFC. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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