TFAP4
Transcription factor AP-4
Also known as: AP-4, bHLHc41, TFAP4_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q01664
- Gene
- TFAP4
- Ensembl
- ENSG00000090447
- Chromosome
- 16
- Canonical length
- 338 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Mitochondria
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]
Canonical amino-acid sequenceUniProt
338 residues, UniProt reviewed canonical sequence.
>Q01664|TFAP4
1 MEYFMVPTQK VPSLQHFRKT EKEVIGGLCS LANIPLTPET QRDQERRIRR EIANSNERRR
61 MQSINAGFQS LKTLIPHTDG EKLSKAAILQ QTAEYIFSLE QEKTRLLQQN TQLKRFIQEL
121 SGSSPKRRRA EDKDEGIGSP DIWEDEKAED LRREMIELRQ QLDKERSVRM MLEEQVRSLE
181 AHMYPEKLKV IAQQVQLQQQ QEQVRLLHQE KLEREQQQLR TQLLPPPAPT HHPTVIVPAP
241 PPPPSHHINV VTMGPSSVIN SVSTSRQNLD TIVQAIQHIE GTQEKQELEE EQRRAVIVKP
301 VRSCPEAPTS DTASDSEASD SDAMDQSREE PSGDGELPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TFAP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.57
- Highest tissue expression
- 14 nTPM
Expression across tissuesHPA
Tissue
- cerebellum: 14 nTPM
- pancreas: 11 nTPM
- skin: 10 nTPM
- cervix: 9.4 nTPM
- ovary: 9.2 nTPM
- vagina: 8.1 nTPM
Single-cell type
- enterocytes: 35 nCPM
- gastric chief cells: 28 nCPM
- enteric stem cells: 26 nCPM
- mucous neck cells: 26 nCPM
- tuft cells: 23 nCPM
- parietal cells: 23 nCPM
Immune cell
- memory B-cell: 0.2 nTPM
- eosinophil: 0.1 nTPM
- MAIT T-cell: 0.1 nTPM
- naive CD8 T-cell: 0.1 nTPM
- total PBMC: 0.1 nTPM
- basophil: 0 nTPM
Brain region
- cerebellum: 9.2 nTPM
- white matter: 8.2 nTPM
- cerebral cortex: 7.8 nTPM
- medulla oblongata: 5.9 nTPM
- pons: 5.8 nTPM
- basal ganglia: 5.6 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.17
- gnomAD pLI
- 1
- gnomAD missense Z
- 1.6
- DepMap mean gene effect
- -0.33
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to dexamethasone stimulus
- DNA damage response, signal transduction by p53 class mediator
- host-mediated activation of viral transcription
- host-mediated suppression of viral transcription
- negative regulation of cell population proliferation
- negative regulation of cyclin-dependent protein serine/threonine kinase activity
- negative regulation of DNA binding
- negative regulation of DNA-templated transcription
- negative regulation of gene expression
- positive regulation of apoptotic process
- positive regulation of DNA-templated transcription
- protein-containing complex assembly
- regulation of mitotic cell cycle phase transition
- regulation of transcription by RNA polymerase II
Molecular functions
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity, RNA polymerase II-specific
- E-box binding
- histone deacetylase binding
- protein homodimerization activity
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- transcription cis-regulatory region binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TFAP4 as an antibody target. Whether an autoantibody or antibody against TFAP4 could matter depends on whether native TFAP4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TFAP4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label TFAP4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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