Seroatlas · Human Serome Atlas

TCFL5

Transcription factor-like 5 protein

Also known as: bHLHe82, CHA, E2BP-1, Figlb, TCFL5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UL49
Gene
TCFL5
Ensembl
ENSG00000101190
Chromosome
20
Canonical length
500 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including negative regulation of transcription by RNA polymerase II; regulation of cell population proliferation; and spermatogenesis. Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

500 residues, UniProt reviewed canonical sequence.

>Q9UL49|TCFL5
     1  MSGPGPREPP PEAGAAGGEA AVEGAGGGDA ALGEPGLSFT TTDLSLVEMT EVEYTQLQHI
    61  LCSHMEAAAD GELETRLNSA LLAAAGPGAG AGGFAAGGQG GAAPVYPVLC PSALAADAPC
   121  LGHIDFQELR MMLLSEAGAA EKTSGGGDGA RARADGAAKE GAGAAAAAAG PDGAPEARAK
   181  PAVRVRLEDR FNSIPAEPPP APRGPEPPEP GGALNNLVTL IRHPSELMNV PLQQQNKCTA
   241  LVKNKTAATT TALQFTYPLF TTNACSTSGN SNLSQTQSSS NSCSVLEAAK HQDIGLPRAF
   301  SFCYQQEIES TKQTLGSRNK VLPEQVWIKV GEAALCKQAL KRNRSRMRQL DTNVERRALG
   361  EIQNVGEGAT ATQGAWQSSE SSQANLGEQA QSGPQGGRSQ RRERHNRMER DRRRRIRICC
   421  DELNLLVPFC NAETDKATTL QWTTAFLKYI QERHGDSLKK EFESVFCGKT GRRLKLTRPD
   481  SLVTCPAQGS LQSSPSMEIK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TCFL5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.68
Highest tissue expression
69 nTPM

Expression across tissuesHPA

Tissue

  • testis: 69 nTPM
  • spinal cord: 66 nTPM
  • midbrain: 50 nTPM
  • basal ganglia: 33 nTPM
  • hippocampal formation: 33 nTPM
  • amygdala: 32 nTPM

Single-cell type

  • late primary spermatocytes: 83 nCPM
  • oligodendrocytes: 38 nCPM
  • melanocytes: 38 nCPM
  • early primary spermatocytes: 33 nCPM
  • epididymal clear cells: 25 nCPM
  • lymphatic endothelial cells: 24 nCPM

Immune cell

  • eosinophil: 0.6 nTPM
  • gdT-cell: 0.6 nTPM
  • neutrophil: 0.5 nTPM
  • MAIT T-cell: 0.4 nTPM
  • memory CD4 T-cell: 0.4 nTPM
  • memory CD8 T-cell: 0.4 nTPM

Brain region

  • white matter: 71 nTPM
  • basal ganglia: 59 nTPM
  • midbrain: 56 nTPM
  • spinal cord: 55 nTPM
  • pons: 51 nTPM
  • medulla oblongata: 50 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TCFL5.

Disease | ImmuneIEDB

Conditions an epitope on TCFL5 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.26
gnomAD pLI
0.99
gnomAD missense Z
1.1
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TCFL5 as an antibody target. Whether an autoantibody or antibody against TCFL5 could matter depends on whether native TCFL5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TCFL5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TCFL5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TCFL5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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