SSX4

Protein identityUniProt · HPA

UniProt accession

O60224

Gene

SSX4

Ensembl

ENSG00000268009

Chromosome

X

Canonical length

188 aa

Protein class

Cancer-related genes, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli

OverviewNCBI Gene

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

188 residues, UniProt reviewed canonical sequence.

>O60224|SSX4
     1  MNGDDAFARR PRDDAQISEK LRKAFDDIAK YFSKKEWEKM KSSEKIVYVY MKLNYEVMTK
    61  LGFKVTLPPF MRSKRAADFH GNDFGNDRNH RNQVERPQMT FGSLQRIFPK IMPKKPAEEE
   121  NGLKEVPEAS GPQNDGKQLC PPGNPSTLEK INKTSGPKRG KHAWTHRLRE RKQLVVYEEI
   181  SDPEEDDE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SSX4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

2.9 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.3

gnomAD pLI

0.52

gnomAD missense Z

-0.42

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of DNA-templated transcription

Cellular components

• nucleus

Protein domainsUniProt · Pfam · InterPro

• Krueppel-associated box
• Ancestral KRAB domain
• SSXRD motif
• Krueppel-associated box domain superfamily
• SSXRD motif

KeywordsUniProt

• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of SSX4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HIF1A