Seroatlas · Human Serome Atlas

SPINK4

Serine protease inhibitor Kazal-type 4

Also known as: ISK4_HUMAN, MGC133107, PEC-60

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60575
Gene
SPINK4
Ensembl
ENSG00000122711
Chromosome
9
Canonical length
86 aa
Protein class
Predicted intracellular proteins, Predicted secreted proteins
Subcellular location
Nucleoplasm,Golgi apparatus,Vesicles
Secretome location
Secreted to digestive system

OverviewNCBI Gene

Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be located in extracellular region. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

86 residues, UniProt reviewed canonical sequence.

>O60575|SPINK4
     1  MAVRQWVIAL ALAALLVVDR EVPVAAGKLP FSRMPICEHM VESPTCSQMS NLVCGTDGLT
    61  YTNECQLCLA RIKTKQDIQI MKDGKC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPINK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
915 nTPM

Expression across tissuesHPA

Tissue

  • rectum: 915 nTPM
  • small intestine: 613 nTPM
  • colon: 597 nTPM
  • duodenum: 247 nTPM
  • retina: 85 nTPM
  • appendix: 79 nTPM

Single-cell type

  • goblet cells: 9,486 nCPM
  • enterocytes: 118 nCPM
  • neuroendocrine cells: 95 nCPM
  • rod photoreceptor cells: 68 nCPM
  • epicardial cells: 64 nCPM
  • foveolar cells: 63 nCPM

Immune cell

  • basophil: 1.8 nTPM
  • intermediate monocyte: 0.3 nTPM
  • classical monocyte: 0.1 nTPM
  • MAIT T-cell: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • eosinophil: 0 nTPM

Brain region

  • cerebellum: 1.5 nTPM
  • cerebral cortex: 0.9 nTPM
  • white matter: 0.6 nTPM
  • medulla oblongata: 0.4 nTPM
  • pons: 0.4 nTPM
  • amygdala: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.19
gnomAD pLI
0.3
gnomAD missense Z
0.34
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPINK4 as an antibody target. Whether an autoantibody or antibody against SPINK4 could matter depends on whether native SPINK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPINK4 is annotated as secreted, so native SPINK4 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SPINK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPINK4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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