Seroatlas · Human Serome Atlas

SPATA4

Spermatogenesis-associated protein 4

Also known as: CFAP178, FAP178, SPAT4_HUMAN, SPEF1B, TSARG2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NEY3
Gene: SPATA4
Ensembl: ENSG00000150628
Chromosome: 4
Canonical length: 305 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable microtubule binding activity. Predicted to be involved in regulation of cytoskeleton organization. Located in cytosol and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

305 residues, UniProt reviewed canonical sequence.

>Q8NEY3|SPATA4
     1  MAAAGQEKGY LTQTAAALDK SPSLSPQLAA PIRGRPKKCL VYPHAPKSSR LSRSVLRWLQ
    61  GLDLSFFPRN INRDFSNGFL IAEIFCIYYP WELELSSFEN GTSLKVKLDN WAQLEKFLAR
   121  KKFKLPKELI HGTIHCKAGV PEILIEEVYT LLTHREIKSI QDDFVNFTDY SYQMRLPLVS
   181  RSTVSKSIKD NIRLSELLSN PNMLTNELKA EFLILLHMLQ RKLGRKLNPE WFDVKPTVGE
   241  VTLNHLPAQA SGRRYNLKVK RGRVVPVLPN IGSGGSSHRE IHVKQAGQHS YYSAMKPIRN
   301  MDKKP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPATA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 130 nTPM

Expression across tissuesHPA

Tissue

testis: 130 nTPM
fallopian tube: 8.2 nTPM
epididymis: 3.4 nTPM
kidney: 2.1 nTPM
retina: 2.1 nTPM
choroid plexus: 1.8 nTPM

Single-cell type

late primary spermatocytes: 1,446 nCPM
early spermatids: 1,012 nCPM
late spermatids: 644 nCPM
early primary spermatocytes: 100 nCPM
respiratory ciliated cells: 92 nCPM
fallopian tube ciliated cells: 61 nCPM

Immune cell

basophil: 1.2 nTPM
naive B-cell: 0.1 nTPM
NK-cell: 0.1 nTPM
non-classical monocyte: 0.1 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM

Brain region

midbrain: 4.8 nTPM
medulla oblongata: 4.3 nTPM
choroid plexus: 3.6 nTPM
spinal cord: 3.3 nTPM
hypothalamus: 3 nTPM
cerebral cortex: 2.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.51
gnomAD pLI: 0
gnomAD missense Z: -0.32
DepMap mean gene effect: 0.14
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of cytoskeleton organization

Molecular functions

microtubule binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Nucleus

InteractionsUniProt · HPA

Protein binding partners of SPATA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

CFAP65

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPATA4 as an antibody target. Whether an autoantibody or antibody against SPATA4 could matter depends on whether native SPATA4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPATA4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPATA4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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