Seroatlas · Human Serome Atlas

SPATA32

Spermatogenesis-associated protein 32

Also known as: AEP2, C17orf46, FLJ25414, SPT32_HUMAN, TEX34, VAD1.2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96LK8
Gene: SPATA32
Ensembl: ENSG00000184361
Chromosome: 17
Canonical length: 384 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable actin binding activity. Predicted to be involved in spermatogenesis. Predicted to be active in perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

384 residues, UniProt reviewed canonical sequence.

>Q96LK8|SPATA32
     1  MGVTGAHGFP CCGKGSVEVA EMRDDLSQHQ IQEEQELEAD MLEQKPQLQV DLDLDPDPDP
    61  DPELEIGQVP ALLESELYPA LKLEAELDTE ANSNEESDFE EPMQLVCKIE SVHSNMGLPT
   121  PQTFRPWSLN SNCRSFTEEN HVSACHHSIS AQTSKHLFWA NKLIQASEHS LQRAINMQLN
   181  NGSAGQPIRS PLREAIPTNA LCSEEQLQIP DAHSAPPTTS SQAPSPLLSS DLPPPIDLTE
   241  LITFASSLAM ASSSRMDLPS LEHMMKAPPQ EALEPSTEPL LTTVEEREPE NHAETLPEKP
   301  REARAPLKSW SQEDKNFAQS YFDFSKPGIK RATIKGQIQL LQPPATSPLL QGSKEDSVPP
   361  GKEKENPLLV KIHFKLSAPT IPEK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPATA32 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.67
Highest tissue expression: 44 nTPM

Expression across tissuesHPA

Tissue

testis: 44 nTPM
duodenum: 0.5 nTPM
small intestine: 0.4 nTPM
appendix: 0.3 nTPM
lymph node: 0.3 nTPM
bone marrow: 0.2 nTPM

Single-cell type

late spermatids: 1,396 nCPM
early spermatids: 388 nCPM
late primary spermatocytes: 41 nCPM
epididymal basal cells: 14 nCPM
pdcs: 8.2 nCPM
innate lymphoid cells: 5.8 nCPM

Immune cell

naive CD4 T-cell: 0.6 nTPM
memory B-cell: 0.3 nTPM
memory CD4 T-cell: 0.3 nTPM
memory CD8 T-cell: 0.3 nTPM
naive B-cell: 0.3 nTPM
naive CD8 T-cell: 0.3 nTPM

Brain region

cerebellum: 0.3 nTPM
cerebral cortex: 0.3 nTPM
hypothalamus: 0.2 nTPM
amygdala: 0.1 nTPM
basal ganglia: 0.1 nTPM
hippocampal formation: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.49
gnomAD pLI: 0
gnomAD missense Z: 0.58
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Protein domainsUniProt · Pfam · InterPro

Spermatogenesis-associated protein 32
Vitamin A-deficiency (VAD) rat model signalling

KeywordsUniProt

Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of SPATA32 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

ACTB

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPATA32 as an antibody target. Whether an autoantibody or antibody against SPATA32 could matter depends on whether native SPATA32 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPATA32 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPATA32 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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