Seroatlas · Human Serome Atlas

SPATA25

Spermatogenesis-associated protein 25

Also known as: C20orf165, dJ337O18.8, SPT25_HUMAN, TSG23

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BR10
Gene: SPATA25
Ensembl: ENSG00000149634
Chromosome: 20
Canonical length: 227 aa
Protein class: Predicted intracellular proteins
Subcellular location: Golgi apparatus

OverviewNCBI Gene

Involved in spermatogenesis. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

227 residues, UniProt reviewed canonical sequence.

>Q9BR10|SPATA25
     1  MSYFRTPQTH PGPLPSGQGG AASPGLSLGL CSPVEPVVVA SGGTGPLSQK AEQVAPAAQA
    61  WGPALAMPQA RGCPGGTSWE TLRKEYSRNC HKFPHVRQLE SLGWDNGYSR SRAPDLGGPS
   121  RPRPLMLCGL SPRVLPVPSE AVGKEASSQP DICILTLAMM IAGIPTVPVP GVREEDLIWA
   181  AQAFMMAHPE PEGAVEGARW EQAHAHTASG KMPLVRSKRG QPPGSCL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPATA25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.71
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

testis: 23 nTPM
skeletal muscle: 9.2 nTPM
thymus: 6.4 nTPM
ovary: 5.1 nTPM
kidney: 5 nTPM
liver: 5 nTPM

Single-cell type

early spermatids: 370 nCPM
late primary spermatocytes: 132 nCPM
late spermatids: 69 nCPM
hofbauer cells: 0.8 nCPM
cardiomyocytes: 0.7 nCPM
macrophages: 0.7 nCPM

Immune cell

memory B-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

cerebellum: 12 nTPM
basal ganglia: 11 nTPM
choroid plexus: 11 nTPM
white matter: 9.4 nTPM
medulla oblongata: 8.9 nTPM
cerebral cortex: 8.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.45
gnomAD pLI: 0
gnomAD missense Z: -0.31
DepMap mean gene effect: 0
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

Spermatogenesis-associated protein 25
Spermatogenesis-associated protein 25

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPATA25 as an antibody target. Whether an autoantibody or antibody against SPATA25 could matter depends on whether native SPATA25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPATA25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPATA25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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