Seroatlas · Human Serome Atlas

SPACA7

Sperm acrosome-associated protein 7

Also known as: C13orf28, SPAC7_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96KW9
Gene
SPACA7
Ensembl
ENSG00000153498
Chromosome
13
Canonical length
195 aa
Protein class
Predicted intracellular proteins, Predicted secreted proteins
Subcellular location
Microtubules,Mitochondria,Acrosome,Equatorial segment,Principal piece,End piece
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

Predicted to act upstream of or within negative regulation of cell adhesion and single fertilization. Located in acrosomal vesicle. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

195 residues, UniProt reviewed canonical sequence.

>Q96KW9|SPACA7
     1  MAVSQGDGTL CFVLLLCCWQ ETELRPRTVI PGSPTEIPFS SKQEDMSELL DEILVQEILD
    61  LNKTTPSEMP STASTLSTPL HAGIDENYQA GGSENYHELL ENLQFSPGIE VKISNDEANA
   121  NANLHGDPSE NYRGPQVSPG SEKSVSSKEK NSKNTQYENL SILDQILQNI GRSSGNIFHK
   181  EQQRTSAQRR SQGSQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPACA7 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.67
Highest tissue expression
84 nTPM

Expression across tissuesHPA

Tissue

  • testis: 84 nTPM
  • bone marrow: 0.5 nTPM
  • salivary gland: 0.3 nTPM
  • liver: 0.2 nTPM
  • lymph node: 0.2 nTPM
  • retina: 0.2 nTPM

Single-cell type

  • early spermatids: 1,323 nCPM
  • late spermatids: 935 nCPM
  • late primary spermatocytes: 553 nCPM
  • sertoli cells: 3.1 nCPM
  • leydig cells: 1.6 nCPM
  • differentiating spermatogonia: 1.2 nCPM

Immune cell

  • neutrophil: 1 nTPM
  • basophil: 0.7 nTPM
  • classical monocyte: 0.2 nTPM
  • eosinophil: 0.2 nTPM
  • gdT-cell: 0.1 nTPM
  • intermediate monocyte: 0.1 nTPM

Brain region

  • cerebellum: 4.5 nTPM
  • choroid plexus: 4.4 nTPM
  • cerebral cortex: 4.2 nTPM
  • basal ganglia: 4 nTPM
  • medulla oblongata: 4 nTPM
  • white matter: 4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.41
gnomAD pLI
0
gnomAD missense Z
0.12
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 1% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Sperm acrosome-associated protein 7
  • Sperm acrosome-associated protein 7

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPACA7 as an antibody target. Whether an autoantibody or antibody against SPACA7 could matter depends on whether native SPACA7 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPACA7 is annotated as secreted, so native SPACA7 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SPACA7 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPACA7. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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