Seroatlas · Human Serome Atlas

SPACA5

Sperm acrosome-associated protein 5

Also known as: dJ54B20.3, LYC5, LYZL5, LYZL5_HUMAN, LYZL5B, PNPK6288, SLLP2, SPACA5B, UNQ6288

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96QH8
Gene
SPACA5
Ensembl
ENSG00000171489
Chromosome
X
Canonical length
159 aa
Protein class
Enzymes, Predicted secreted proteins
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

Predicted to enable lysozyme activity. Predicted to be involved in fusion of sperm to egg plasma membrane involved in single fertilization. Predicted to be located in extracellular region. Predicted to be active in acrosomal vesicle and sperm flagellum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

159 residues, UniProt reviewed canonical sequence.

>Q96QH8|SPACA5
     1  MKAWGTVVVT LATLMVVTVD AKIYERCELA ARLERAGLNG YKGYGVGDWL CMAHYESGFD
    61  TAFVDHNPDG SSEYGIFQLN SAWWCDNGIT PTKNLCHMDC HDLLNRHILD DIRCAKQIVS
   121  SQNGLSAWTS WRLHCSGHDL SEWLKGCDMH VKIDPKIHP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPACA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
34 nTPM

Expression across tissuesHPA

Tissue

  • testis: 34 nTPM
  • choroid plexus: 0.3 nTPM
  • epididymis: 0.2 nTPM
  • midbrain: 0.2 nTPM
  • skin: 0.2 nTPM
  • amygdala: 0.1 nTPM

Single-cell type

  • early spermatids: 0.3 nCPM
  • cardiomyocytes: 0.2 nCPM
  • adipocytes: 0 nCPM
  • adrenal cortex cells: 0 nCPM
  • adrenal medulla cells: 0 nCPM
  • alveolar cells type 1: 0 nCPM

Immune cell

  • T-reg: 0.3 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • cerebral cortex: 0.8 nTPM
  • midbrain: 0.6 nTPM
  • pons: 0.6 nTPM
  • thalamus: 0.6 nTPM
  • cerebellum: 0.5 nTPM
  • hypothalamus: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.71
gnomAD pLI
0.4
gnomAD missense Z
0.85

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPACA5 as an antibody target. Whether an autoantibody or antibody against SPACA5 could matter depends on whether native SPACA5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPACA5 is annotated as secreted, so native SPACA5 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SPACA5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPACA5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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