Seroatlas · Human Serome Atlas

SMYD5

Protein-lysine N-trimethyltransferase SMYD5

Also known as: NN8-4AG, RAI15, RRG1, SMYD5_HUMAN, ZMYND23

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6GMV2
Gene: SMYD5
Ensembl: ENSG00000135632
Chromosome: 2
Canonical length: 418 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables protein-lysine N-methyltransferase activity. Involved in positive regulation of cytoplasmic translational elongation; regulation of stem cell differentiation; and transposable element silencing by heterochromatin formation. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

418 residues, UniProt reviewed canonical sequence.

>Q6GMV2|SMYD5
     1  MAASMCDVFS FCVGVAGRAR VSVEVRFVSS AKGKGLFATQ LIRKGETIFV ERPLVAAQFL
    61  WNALYRYRAC DHCLRALEKA EENAQRLTGK PGQVLPHPEL CTVRKDLHQN CPHCQVMYCS
   121  AECRLAATEQ YHQVLCPGPS QDDPLHPLNK LQEAWRSIHY PPETASIMLM ARMVATVKQA
   181  KDKDRWIRLF SQFCNKTANE EEEIVHKLLG DKFKGQLELL RRLFTEALYE EAVSQWFTPD
   241  GFRSLFALVG TNGQGIGTSS LSQWVHACDT LELKPQDREQ LDAFIDQLYK DIEAATGEFL
   301  NCEGSGLFVL QSCCNHSCVP NAETSFPENN FLLHVTALED IKPGEEICIS YLDCCQRERS
   361  RHSRHKILRE NYLFVCSCPK CLAEADEPNV TSEEEEEEEE EEEGEPEDAE LGDEMTDV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SMYD5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

basal ganglia: 25 nTPM
cerebral cortex: 24 nTPM
ovary: 22 nTPM
cerebellum: 19 nTPM
hypothalamus: 19 nTPM
kidney: 19 nTPM

Single-cell type

migrating cytotrophoblasts: 14 nCPM
esophageal basal cells: 13 nCPM
differentiating spermatogonia: 12 nCPM
extravillous trophoblasts: 12 nCPM
gonadotrophs: 11 nCPM
cytotrophoblasts: 10 nCPM

Immune cell

MAIT T-cell: 11 nTPM
naive CD8 T-cell: 7.8 nTPM
memory CD4 T-cell: 7 nTPM
naive CD4 T-cell: 7 nTPM
memory CD8 T-cell: 6.8 nTPM
naive B-cell: 6.2 nTPM

Brain region

pons: 30 nTPM
hypothalamus: 28 nTPM
cerebral cortex: 28 nTPM
basal ganglia: 27 nTPM
amygdala: 26 nTPM
medulla oblongata: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.57
gnomAD pLI: 0.01
gnomAD missense Z: 1.19
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SMYD5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SMYD5 as an antibody target. Whether an autoantibody or antibody against SMYD5 could matter depends on whether native SMYD5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SMYD5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SMYD5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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