Seroatlas · Human Serome Atlas

SMYD4

Protein-lysine N-methyltransferase SMYD4

Also known as: KIAA1936, SMYD4_HUMAN, ZMYND21

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IYR2
Gene: SMYD4
Ensembl: ENSG00000186532
Chromosome: 17
Canonical length: 804 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Golgi apparatus,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable histone deacetylase binding activity. Involved in heart development. Predicted to be active in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

804 residues, UniProt reviewed canonical sequence.

>Q8IYR2|SMYD4
     1  MDLPVDEWKS YLLQKWASLP TSVQVTISTA ETLRDIFLHS SSLLQPEDEL FLKRLSKGYL
    61  VGKDSDAPLF YREEGNKKFQ EKDYTGAAVL YSKGVSHSRP NTEDMSLCHA NRSAALFHLG
   121  QYETCLKDIN RAQTHGYPER LQPKIMLRKA ECLVALGRLQ EASQTISDLE RNFTATPALA
   181  DVLPQTLQRN LHRLKMKMQE KDSLTESFPA ALAKTLEDAA LREENEQLSN ASSSIGLCVD
   241  PLKGRCLVAT KDILPGELLV QEDAFVSVLN PGELPPPHHG LDSKWDTRVT NGDLYCHRCL
   301  KHTLATVPCD GCSYAKYCSQ ECLQQAWELY HRTECPLGGL LLTLGVFCHI ALRLTLLVGF
   361  EDVRKIITKL CDKISNKDIC LPESNNQVKT LNYGLGESEK NGNIVETPIP GCDINGKYEN
   421  NYNAVFNLLP HTENHSPEHK FLCALCVSAL CRQLEAASLQ AIPTERIVNS SQLKAAVTPE
   481  LCPDVTIWGV AMLRHMLQLQ CNAQAMTTIQ HTGPKGSIVT DSRQVRLATG IFPVISLLNH
   541  SCSPNTSVSF ISTVATIRAS QRIRKGQEIL HCYGPHKSRM GVAERQQKLR SQYFFDCACP
   601  ACQTEAHRMA AGPRWEAFCC NSCGAPMQGD DVLRCGSRSC AESAVSRDHL VSRLQDLQQQ
   661  VRVAQKLLRD GELERAVQRL SGCQRDAESF LWAEHAVVGE IADGLARACA ALGDWQKSAT
   721  HLQRSLYVVE VRHGPSSVEM GHELFKLAQI FFNGFAVPEA LSTIQKAEEV LSLHCGPWDD
   781  EIQELQKMKS CLLDLPPTPV GPAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SMYD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 13 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 13 nTPM
tongue: 9.5 nTPM
ovary: 9 nTPM
blood vessel: 7.9 nTPM
retina: 7.2 nTPM
urinary bladder: 7.2 nTPM

Single-cell type

myonuclei: 79 nCPM
epididymal basal cells: 60 nCPM
thymic myoid cells: 56 nCPM
kupffer cells: 55 nCPM
epididymal clear cells: 49 nCPM
ovarian stromal cells: 48 nCPM

Immune cell

basophil: 12 nTPM
gdT-cell: 5.1 nTPM
naive CD8 T-cell: 5 nTPM
plasmacytoid DC: 4.5 nTPM
MAIT T-cell: 4.3 nTPM
memory B-cell: 4.1 nTPM

Brain region

cerebellum: 26 nTPM
white matter: 21 nTPM
cerebral cortex: 19 nTPM
thalamus: 18 nTPM
amygdala: 18 nTPM
pons: 18 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SMYD4.

Disease | ImmuneIEDB

Conditions an epitope on SMYD4 was assayed in.

human immunodeficiency virus infectious disease T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.08
gnomAD pLI: 0
gnomAD missense Z: -0.01
DepMap mean gene effect: -0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

SET domain
Zinc finger, MYND-type
Tetratricopeptide-like helical domain superfamily
SET domain superfamily
SET domain
MYND finger
SMYD4, SET domain
SET and MYND domain-containing protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SMYD4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

HDAC1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SMYD4 as an antibody target. Whether an autoantibody or antibody against SMYD4 could matter depends on whether native SMYD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SMYD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SMYD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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