Seroatlas · Human Serome Atlas

SMIM20

Small integral membrane protein 20

Also known as: C4orf52, MITRAC7, PNX, SIM20_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N5G0
Gene
SMIM20
Ensembl
ENSG00000250317
Chromosome
4
Canonical length
67 aa
Protein class
Predicted membrane proteins, Predicted secreted proteins
Subcellular location
Nucleoplasm,Nucleoli,Cytosol
Secretome location
Secreted to blood

OverviewNCBI Gene

Involved in mitochondrial cytochrome c oxidase assembly. Located in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

67 residues, UniProt reviewed canonical sequence.

>Q8N5G0|SMIM20
     1  MSRNLRTALI FGGFISLIGA AFYPIYFRPL MRLEEYKKEQ AINRAGIVQE DVQPPGLKVW
    61  SDPFGRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SMIM20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.49
Highest tissue expression
92 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 92 nTPM
  • epididymis: 72 nTPM
  • tongue: 60 nTPM
  • kidney: 54 nTPM
  • choroid plexus: 47 nTPM
  • heart muscle: 45 nTPM

Single-cell type

  • epididymal principal cells: 221 nCPM
  • parietal cells: 134 nCPM
  • kupffer cells: 119 nCPM
  • enterocytes: 116 nCPM
  • decidual stromal cells: 98 nCPM
  • plasma cells: 92 nCPM

Immune cell

  • naive CD4 T-cell: 52 nTPM
  • naive B-cell: 50 nTPM
  • memory B-cell: 49 nTPM
  • T-reg: 44 nTPM
  • naive CD8 T-cell: 42 nTPM
  • plasmacytoid DC: 40 nTPM

Brain region

  • choroid plexus: 23 nTPM
  • white matter: 22 nTPM
  • hypothalamus: 20 nTPM
  • cerebellum: 19 nTPM
  • spinal cord: 19 nTPM
  • basal ganglia: 18 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.1
gnomAD pLI
0.58
gnomAD missense Z
0.63
DepMap mean gene effect
0.05
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • MITRAC7/Phoenixin
  • MITRAC7/Phoenixin family

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SMIM20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SMIM20 as an antibody target. Whether an autoantibody or antibody against SMIM20 could matter depends on whether native SMIM20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SMIM20 is annotated as secreted, so native SMIM20 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label SMIM20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SMIM20. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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