Seroatlas · Human Serome Atlas

SETD9

SET domain-containing protein 9

Also known as: C5orf35, MGC33648, SETD9_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NE22
Gene: SETD9
Ensembl: ENSG00000155542
Chromosome: 5
Canonical length: 299 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable lysine N-methyltransferase activity. Predicted to be involved in regulation of signal transduction by p53 class mediator. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

299 residues, UniProt reviewed canonical sequence.

>Q8NE22|SETD9
     1  MPGRLLRGLW QRWRRYKYRF VPWIALNLSH NPRTLRYVPE ESKDKVISDE DVLGTLLKVF
    61  QALFLNDFNK QSEILSMLPE SVKSKYQDLL AVEHQGVKLL ENRHQQQSTF KPEEILYKTL
   121  GFSVAQATSS LISAGKGVFV TKGLVPKGAV VSMYPGTVYQ KYEPIFFQSI GNPFIFRCLD
   181  GVLIDGNDKG ISKVVYRSCN GRDRLGPLKM SDSTWLTSEI HNPLAVGQYV NNCSNDRAAN
   241  VCYQEFDVPA VFPIELKQYL PNIAYSYDKQ SPLRCVVLVA LRDINQGEEL FSNYYTIVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SETD9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

testis: 18 nTPM
liver: 9.3 nTPM
tongue: 6.7 nTPM
small intestine: 6.3 nTPM
salivary gland: 5.9 nTPM
kidney: 5.8 nTPM

Single-cell type

early spermatids: 754 nCPM
late spermatids: 482 nCPM
late primary spermatocytes: 415 nCPM
early primary spermatocytes: 58 nCPM
breast secretory cells: 50 nCPM
colonocytes: 47 nCPM

Immune cell

basophil: 5.2 nTPM
non-classical monocyte: 3.2 nTPM
eosinophil: 1.9 nTPM
neutrophil: 1.8 nTPM
classical monocyte: 1 nTPM
intermediate monocyte: 0.9 nTPM

Brain region

white matter: 2.8 nTPM
medulla oblongata: 2.4 nTPM
pons: 2.1 nTPM
spinal cord: 2.1 nTPM
basal ganglia: 2 nTPM
hypothalamus: 2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.96
gnomAD pLI: 0
gnomAD missense Z: 0.48
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

lysine N-methyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

SET domain
SET domain superfamily
SET domain-containing protein 9

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SETD9 as an antibody target. Whether an autoantibody or antibody against SETD9 could matter depends on whether native SETD9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SETD9 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SETD9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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