Seroatlas · Human Serome Atlas

SETD4

SET domain-containing protein 4

Also known as: C21orf18, C21orf27, SETD4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NVD3
Gene
SETD4
Ensembl
ENSG00000185917
Chromosome
21
Canonical length
440 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nuclear speckles

OverviewNCBI Gene

Enables histone H4K20 methyltransferase activity. Predicted to be involved in several processes, including positive regulation of inflammatory response; positive regulation of macromolecule biosynthetic process; and regulation of cell proliferation in bone marrow. Located in cytosol and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

440 residues, UniProt reviewed canonical sequence.

>Q9NVD3|SETD4
     1  MQKGKGRTSR IRRRKLCGSS ESRGVNESHK SEFIELRKWL KARKFQDSNL APACFPGTGR
    61  GLMSQTSLQE GQMIISLPES CLLTTDTVIR SYLGAYITKW KPPPSPLLAL CTFLVSEKHA
   121  GHRSLWKPYL EILPKAYTCP VCLEPEVVNL LPKSLKAKAE EQRAHVQEFF ASSRDFFSSL
   181  QPLFAEAVDS IFSYSALLWA WCTVNTRAVY LRPRQRECLS AEPDTCALAP YLDLLNHSPH
   241  VQVKAAFNEE THSYEIRTTS RWRKHEEVFI CYGPHDNQRL FLEYGFVSVH NPHACVYVSR
   301  EILVKYLPST DKQMDKKISI LKDHGYIENL TFGWDGPSWR LLTALKLLCL EAEKFTCWKK
   361  VLLGEVISDT NEKTSLDIAQ KICYYFIEET NAVLQKVSHM KDEKEALINQ LTLVESLWTE
   421  ELKILRASAE TLHSLQTAFT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SETD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
22 nTPM

Expression across tissuesHPA

Tissue

  • testis: 22 nTPM
  • choroid plexus: 16 nTPM
  • skin: 13 nTPM
  • cerebral cortex: 11 nTPM
  • duodenum: 11 nTPM
  • epididymis: 11 nTPM

Single-cell type

  • late primary spermatocytes: 115 nCPM
  • choroid plexus epithelial cells: 67 nCPM
  • early primary spermatocytes: 47 nCPM
  • respiratory ciliated cells: 41 nCPM
  • ependymal cells: 37 nCPM
  • early spermatids: 34 nCPM

Immune cell

  • memory B-cell: 16 nTPM
  • plasmacytoid DC: 16 nTPM
  • myeloid DC: 15 nTPM
  • naive B-cell: 13 nTPM
  • T-reg: 13 nTPM
  • classical monocyte: 13 nTPM

Brain region

  • choroid plexus: 34 nTPM
  • white matter: 25 nTPM
  • hypothalamus: 23 nTPM
  • cerebral cortex: 22 nTPM
  • basal ganglia: 21 nTPM
  • pons: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.25
gnomAD pLI
0
gnomAD missense Z
0.34
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SETD4 as an antibody target. Whether an autoantibody or antibody against SETD4 could matter depends on whether native SETD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SETD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SETD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SETD4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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