SETD3

Protein identityUniProt · HPA

UniProt accession

Q86TU7

Gene

SETD3

Ensembl

ENSG00000183576

Chromosome

14

Canonical length

594 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

594 residues, UniProt reviewed canonical sequence.

>Q86TU7|SETD3
     1  MGKKSRVKTQ KSGTGATATV SPKEILNLTS ELLQKCSSPA PGPGKEWEEY VQIRTLVEKI
    61  RKKQKGLSVT FDGKREDYFP DLMKWASENG ASVEGFEMVN FKEEGFGLRA TRDIKAEELF
   121  LWVPRKLLMT VESAKNSVLG PLYSQDRILQ AMGNIALAFH LLCERASPNS FWQPYIQTLP
   181  SEYDTPLYFE EDEVRYLQST QAIHDVFSQY KNTARQYAYF YKVIQTHPHA NKLPLKDSFT
   241  YEDYRWAVSS VMTRQNQIPT EDGSRVTLAL IPLWDMCNHT NGLITTGYNL EDDRCECVAL
   301  QDFRAGEQIY IFYGTRSNAE FVIHSGFFFD NNSHDRVKIK LGVSKSDRLY AMKAEVLARA
   361  GIPTSSVFAL HFTEPPISAQ LLAFLRVFCM TEEELKEHLL GDSAIDRIFT LGNSEFPVSW
   421  DNEVKLWTFL EDRASLLLKT YKTTIEEDKS VLKNHDLSVR AKMAIKLRLG EKEILEKAVK
   481  SAAVNREYYR QQMEEKAPLP KYEESNLGLL ESSVGDSRLP LVLRNLEEEA GVQDALNIRE
   541  AISKAKATEN GLVNGENSIP NGTRSENESL NQESKRAVED AKGSSSDSTA GVKE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SETD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

163 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.02

gnomAD missense Z

1.45

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• actin modification
• positive regulation of DNA-templated transcription
• positive regulation of muscle cell differentiation
• positive regulation of transcription by RNA polymerase II
• peptidyl-histidine methylation
• regulation of uterine smooth muscle contraction

Molecular functions

• actin binding
• histone H3K36 methyltransferase activity
• histone H3K4 methyltransferase activity
• protein-L-histidine N-tele-methyltransferase activity
• RNA polymerase II-specific DNA-binding transcription factor binding
• transcription coactivator activity

Cellular components

• chromatin
• cytoplasm
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• SET domain
• Rubisco LSMT, substrate-binding domain
• Rubisco LSMT, substrate-binding domain superfamily
• SET domain superfamily
• SETD3/SETD6 methyltransferase
• SET domain
• Rubisco LSMT substrate-binding
• Actin-histidine N-methyltransferase SETD3
• SETD3, SET domain

KeywordsUniProt

• Actin-binding
• Cytoplasm
• Methyltransferase
• Nucleus
• Phosphoprotein
• S-adenosyl-L-methionine
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of SETD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MYOD1