Seroatlas · Human Serome Atlas

SCAF1

Splicing factor, arginine/serine-rich 19

Also known as: FLJ00034, SFR19_HUMAN, SR-A1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H7N4
Gene: SCAF1
Ensembl: ENSG00000126461
Chromosome: 19
Canonical length: 1312 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables RNA polymerase II C-terminal domain binding activity. Predicted to be involved in RNA splicing; mRNA processing; and transcription by RNA polymerase II. Predicted to be located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1312 residues, UniProt reviewed canonical sequence.

>Q9H7N4|SCAF1
     1  MEEEDESRGK TEESGEDRGD GPPDRDPTLS PSAFILRAIQ QAVGSSLQGD LPNDKDGSRC
    61  HGLRWRRCRS PRSEPRSQES GGTDTATVLD MATDSFLAGL VSVLDPPDTW VPSRLDLRPG
   121  ESEDMLELVA EVRIGDRDPI PLPVPSLLPR LRAWRTGKTV SPQSNSSRPT CARHLTLGTG
   181  DGGPAPPPAP SSASSSPSPS PSSSSPSPPP PPPPPAPPAP PAPRFDIYDP FHPTDEAYSP
   241  PPAPEQKYDP FEPTGSNPSS SAGTPSPEEE EEEEEEEEEE EEDEEEEEGL SQSISRISET
   301  LAGIYDDNSL SQDFPGDESP RPDAQPTQPT PAPGTPPQVD STRADGAMRR RVFVVGTEAE
   361  ACREGKVSVE VVTAGGAALP PPLLPPGDSE IEEGEIVQPE EEPRLALSLF RPGGRAARPT
   421  PAASATPTAQ PLPQPPAPRA PEGDDFLSLH AESDGEGALQ VDLGEPAPAP PAADSRWGGL
   481  DLRRKILTQR RERYRQRSPS PAPAPAPAAA AGPPTRKKSR RERKRSGEAK EAASSSSGTQ
   541  PAPPAPASPW DSKKHRSRDR KPGSHASSSA RRRSRSRSRS RSTRRRSRST DRRRGGSRRS
   601  RSREKRRRRR RSASPPPATS SSSSSRRERH RGKHRDGGGS KKKKKRSRSR GEKRSGDGSE
   661  KAPAPAPPPS GSTSCGDRDS RRRGAVPPSI QDLTDHDLFA IKRTITVGRL DKSDPRGPSP
   721  APASSPKREV LYDSEGLSGE ERGGKSSQKD RRRSGAASSS SSSREKGSRR KALDGGDRDR
   781  DRDRDRDRDR SSKKARPPKE SAPSSGPPPK PPVSSGSGSS SSSSSCSSRK VKLQSKVAVL
   841  IREGVSSTTP AKDAASAGLG SIGVKFSRDR ESRSPFLKPD ERAPTEMAKA APGSTKPKKT
   901  KVKAKAGAKK TKGTKGKTKP SKTRKKVRSG GGSGGSGGQV SLKKSKADSC SQAAGTKGAE
   961  ETSWSGEERA AKVPSTPPPK AAPPPPALTP DSQTVDSSCK TPEVSFLPEE ATEEAGVRGG
  1021  AEEEEEEEEE EEEEEEEEEQ QPATTTATST AAAAPSTAPS AGSTAGDSGA EDGPASRVSQ
  1081  LPTLPPPMPW NLPAGVDCTT SGVLALTALL FKMEEANLAS RAKAQELIQA TNQILSHRKP
  1141  PSSLGMTPAP VPTSLGLPPG PSSYLLPGSL PLGGCGSTPP TPTGLAATSD KREGSSSSEG
  1201  RGDTDKYLKK LHTQERAVEE VKLAIKPYYQ KKDITKEEYK DILRKAVHKI CHSKSGEINP
  1261  VKVSNLVRAY VQRYRYFRKH GRKPGDPPGP PRPPKEPGPP DKGGPGLPLP PL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SCAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.68
Highest tissue expression: 47 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 47 nTPM
skeletal muscle: 44 nTPM
cerebellum: 40 nTPM
amygdala: 37 nTPM
hippocampal formation: 37 nTPM
basal ganglia: 33 nTPM

Single-cell type

late spermatids: 175 nCPM
late primary spermatocytes: 54 nCPM
alveolar cells type 1: 47 nCPM
colonocytes: 32 nCPM
fallopian tube ciliated cells: 32 nCPM
enterocytes: 31 nCPM

Immune cell

neutrophil: 3.6 nTPM
memory B-cell: 2.8 nTPM
plasmacytoid DC: 2.4 nTPM
naive B-cell: 2.1 nTPM
basophil: 2 nTPM
gdT-cell: 1.9 nTPM

Brain region

medulla oblongata: 126 nTPM
cerebral cortex: 123 nTPM
amygdala: 111 nTPM
pons: 110 nTPM
midbrain: 109 nTPM
hippocampal formation: 108 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.25
gnomAD pLI: 1
gnomAD missense Z: 1.4
DepMap mean gene effect: -0.27
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

SFR19-like, C-terminal domain
SCAF11-like, C-terminal domain
Splicing factor, arginine/serine-rich 19

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SCAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

POLR2A

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SCAF1 as an antibody target. Whether an autoantibody or antibody against SCAF1 could matter depends on whether native SCAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SCAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SCAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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