Seroatlas · Human Serome Atlas

RPUSD1

RNA pseudouridylate synthase domain-containing protein 1

Also known as: C16orf40, MGC19600, RLUCL, RUSD1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UJJ7
Gene: RPUSD1
Ensembl: ENSG00000007376
Chromosome: 16
Canonical length: 312 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus,Cell Junctions,Cytosol

OverviewNCBI Gene

Predicted to enable pseudouridine synthase activity. Predicted to be involved in enzyme-directed rRNA pseudouridine synthesis. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

312 residues, UniProt reviewed canonical sequence.

>Q9UJJ7|RPUSD1
     1  MEPGSVENLS IVYRSRDFLV VNKHWDVRID SKAWRETLTL QKQLRYRFPE LADPDTCYGF
    61  RFCHQLDFST SGALCVALNK AAAGSAYRCF KERRVTKAYL ALLRGHIQES RVTISHAIGR
   121  NSTEGRAHTM CIEGSQGCEN PKPSLTDLVV LEHGLYAGDP VSKVLLKPLT GRTHQLRVHC
   181  SALGHPVVGD LTYGEVSGRE DRPFRMMLHA FYLRIPTDTE CVEVCTPDPF LPSLDACWSP
   241  HTLLQSLDQL VQALRATPDP DPEDRGPRPG SPSALLPGPG RPPPPPTKPP ETEAQRGPCL
   301  QWLSEWTLEP DS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPUSD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 23 nTPM
spleen: 21 nTPM
cerebellum: 18 nTPM
esophagus: 18 nTPM
skin: 17 nTPM
stomach: 16 nTPM

Single-cell type

esophageal basal cells: 36 nCPM
migrating cytotrophoblasts: 30 nCPM
extravillous trophoblasts: 30 nCPM
epididymal principal cells: 26 nCPM
retinal bipolar cells: 26 nCPM
megakaryocytes: 25 nCPM

Immune cell

myeloid DC: 17 nTPM
basophil: 16 nTPM
plasmacytoid DC: 16 nTPM
classical monocyte: 15 nTPM
T-reg: 15 nTPM
memory CD8 T-cell: 14 nTPM

Brain region

cerebral cortex: 29 nTPM
medulla oblongata: 23 nTPM
hippocampal formation: 22 nTPM
thalamus: 21 nTPM
amygdala: 21 nTPM
basal ganglia: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.89
gnomAD pLI: 0
gnomAD missense Z: -1.49
DepMap mean gene effect: 0.16
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

enzyme-directed rRNA pseudouridine synthesis

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Acetylation

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPUSD1 as an antibody target. Whether an autoantibody or antibody against RPUSD1 could matter depends on whether native RPUSD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPUSD1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPUSD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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