Seroatlas · Human Serome Atlas

RNF224

RING finger protein 224

Also known as: RN224_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P0DH78
Gene
RNF224
Ensembl
ENSG00000233198
Chromosome
9
Canonical length
156 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Predicted to enable zinc ion binding activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

156 residues, UniProt reviewed canonical sequence.

>P0DH78|RNF224
     1  MQDAAAGGPP GLGGGGPPEE RTDCIICCSA YDLSGHLPRR LYCGHTFCQA CVRRLDTPAP
    61  EQRWIPCPQC RQSTPTPRGG VAMLDLDLAA FLAVKAEREP ARLEPLPLTS LKGSAITRQP
   121  AGLCPALGPQ PHFPQPRYCC WGCGSLCCPP LGSPEV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF224 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
5 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 5 nTPM
  • esophagus: 3.5 nTPM
  • kidney: 1.9 nTPM
  • salivary gland: 1.3 nTPM
  • fallopian tube: 1.1 nTPM
  • testis: 1 nTPM

Single-cell type

  • esophageal apical cells: 23 nCPM
  • esophageal suprabasal cells: 3.4 nCPM
  • gonadotrophs: 3 nCPM
  • fallopian tube ciliated cells: 2.4 nCPM
  • mesothelial cells: 1.9 nCPM
  • salivary duct cells: 1.5 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • midbrain: 5.5 nTPM
  • cerebral cortex: 4.2 nTPM
  • medulla oblongata: 4 nTPM
  • spinal cord: 4 nTPM
  • thalamus: 3.4 nTPM
  • white matter: 3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.76
gnomAD pLI
0.04
gnomAD missense Z
-0.23
DepMap mean gene effect
-0.11
DepMap dependency class
selective

OntologyGO

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF224 as an antibody target. Whether an autoantibody or antibody against RNF224 could matter depends on whether native RNF224 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF224 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF224 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF224. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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