Seroatlas · Human Serome Atlas

RNF223

RING finger protein 223

Also known as: RN223_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: E7ERA6
Gene: RNF223
Ensembl: ENSG00000237330
Chromosome: 1
Canonical length: 249 aa
Protein class: Predicted membrane proteins

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in protein ubiquitination. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

249 residues, UniProt reviewed canonical sequence.

>E7ERA6|RNF223
     1  MSSGQQVWHT AVPPPRRSSS IASMPRSPSS AGSPRSPGTP GSERVASPLE CSICFSGYDN
    61  IFKTPKELSC THVFCLECLA RLAAAQPVGR PGGEAVPCPF CRQPTAVPPA GAPALCTSRQ
   121  LQARMPAHLR REEPVWLEGT KLCCQPLPTT PGREPGFVCV DVGLSKPAEP PAPARDPAPR
   181  RGRLARCWAR CRDWRRMALV SALLLMLFCV ALWPVQCALK TGNLRCLPLP PRPPATSTAA
   241  SPLGPLTDN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF223 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

esophagus: 26 nTPM
vagina: 12 nTPM
cervix: 11 nTPM
salivary gland: 3.7 nTPM
stomach: 3.6 nTPM
urinary bladder: 3.2 nTPM

Single-cell type

esophageal apical cells: 441 nCPM
endometrial secretory cells: 67 nCPM
urothelial cells: 57 nCPM
breast hormone-responsive cells: 56 nCPM
esophageal suprabasal cells: 42 nCPM
breast secretory cells: 39 nCPM

Immune cell

basophil: 0.3 nTPM
neutrophil: 0.1 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

cerebellum: 4.3 nTPM
pons: 4.3 nTPM
cerebral cortex: 3.5 nTPM
medulla oblongata: 3.4 nTPM
hypothalamus: 2.8 nTPM
white matter: 2.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.87
gnomAD pLI: 0.03
gnomAD missense Z: -0.55
DepMap mean gene effect: -0.13
DepMap dependency class: selective

OntologyGO

Biological processes

protein ubiquitination

Molecular functions

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF223 as an antibody target. Whether an autoantibody or antibody against RNF223 could matter depends on whether native RNF223 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF223 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF223 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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