Seroatlas · Human Serome Atlas

RNF148

RING finger protein 148

Also known as: MGC35222, RN148_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N7C7
Gene: RNF148
Ensembl: ENSG00000235631
Chromosome: 7
Canonical length: 305 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ubiquitin-dependent protein catabolic process. Predicted to be located in membrane. Predicted to be active in Golgi apparatus; endoplasmic reticulum; and late endosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

305 residues, UniProt reviewed canonical sequence.

>Q8N7C7|RNF148
     1  MSFLRITPST HSSVSSGLLR LSIFLLLSFP DSNGKAIWTA HLNITFQVGN EITSELGESG
    61  VFGNHSPLER VSGVVALPEG WNQNACHPLT NFSRPKQADS WLALIERGGC TFTHKINVAA
   121  EKGANGVIIY NYQGTGSKVF PMSHQGTENI VAVMISNLKG MEILHSIQKG VYVTVIIEVG
   181  RMHMQWVSHY IMYLFTFLAA TIAYFYLDCV WRLTPRVPNS FTRRRSQIKT DVKKAIDQLQ
   241  LRVLKEGDEE LDLNEDNCVV CFDTYKPQDV VRILTCKHFF HKACIDPWLL AHRTCPMCKC
   301  DILKT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF148 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 48 nTPM

Expression across tissuesHPA

Tissue

testis: 48 nTPM
cerebellum: 0.5 nTPM
endometrium: 0.5 nTPM
lung: 0.4 nTPM
prostate: 0.4 nTPM
salivary gland: 0.4 nTPM

Single-cell type

early spermatids: 8.7 nCPM
late primary spermatocytes: 3.9 nCPM
late spermatids: 2 nCPM
leydig cells: 0.2 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 22 nTPM
cerebral cortex: 5.7 nTPM
white matter: 3.6 nTPM
hypothalamus: 2.6 nTPM
pons: 2.5 nTPM
midbrain: 2.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.56
gnomAD pLI: 0
gnomAD missense Z: -1.52
DepMap mean gene effect: 0.04
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

ubiquitin-dependent protein catabolic process

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF148 as an antibody target. Whether an autoantibody or antibody against RNF148 could matter depends on whether native RNF148 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF148 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF148 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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