Seroatlas · Human Serome Atlas

RNASE9

Inactive ribonuclease-like protein 9

Also known as: h461, RAK1, RNAS9_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P60153
Gene
RNASE9
Ensembl
ENSG00000188655
Chromosome
14
Canonical length
205 aa
Protein class
Predicted secreted proteins
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

Predicted to enable nucleic acid binding activity. Predicted to be involved in defense response to Gram-positive bacterium. Predicted to act upstream of or within positive regulation of flagellated sperm motility involved in capacitation. Predicted to be located in extracellular region. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

205 residues, UniProt reviewed canonical sequence.

>P60153|RNASE9
     1  MMRTLITTHP LPLLLLPQQL LQLVQFQEVD TDFDFPEEDK KEEFEECLEK FFSTGPARPP
    61  TKEKVKRRVL IEPGMPLNHI EYCNHEIMGK NVYYKHRWVA EHYFLLMQYD ELQKICYNRF
   121  VPCKNGIRKC NRSKGLVEGV YCNLTEAFEI PACKYESLYR KGYVLITCSW QNEMQKRIPH
   181  TINDLVEPPE HRSFLSEDGV FVISP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNASE9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
93 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 93 nTPM
  • adipose tissue: 0 nTPM
  • adrenal gland: 0 nTPM
  • amygdala: 0 nTPM
  • appendix: 0 nTPM
  • basal ganglia: 0 nTPM

Single-cell type

  • epididymal principal cells: 143 nCPM
  • epididymal basal cells: 2 nCPM
  • epididymal clear cells: 2 nCPM
  • mast cells: 1 nCPM
  • epididymal efferent duct ciliated cells: 0.4 nCPM
  • pericytes: 0.4 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • amygdala: 0 nTPM
  • basal ganglia: 0 nTPM
  • cerebellum: 0 nTPM
  • cerebral cortex: 0 nTPM
  • choroid plexus: 0 nTPM
  • hippocampal formation: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.83
gnomAD pLI
0.34
gnomAD missense Z
-0.13
DepMap mean gene effect
0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNASE9 as an antibody target. Whether an autoantibody or antibody against RNASE9 could matter depends on whether native RNASE9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNASE9 is annotated as secreted, so native RNASE9 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label RNASE9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNASE9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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