RGN

Protein identityUniProt · HPA

UniProt accession

Q15493

Gene

RGN

Ensembl

ENSG00000130988

Chromosome

X

Canonical length

299 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

299 residues, UniProt reviewed canonical sequence.

>Q15493|RGN
     1  MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ RVTMDAPVSS
    61  VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN RFNDGKVDPA GRYFAGTMAE
   121  ETAPAVLERH QGALYSLFPD HHVKKYFDQV DISNGLDWSL DHKIFYYIDS LSYSVDAFDY
   181  DLQTGQISNR RSVYKLEKEE QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL
   241  PVDKTTSCCF GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RGN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.21

Highest tissue expression

359 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for RGN from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.99

gnomAD pLI

0.02

gnomAD missense Z

-0.91

DepMap mean gene effect

-0.13

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular calcium ion homeostasis
• positive regulation of ATP-dependent activity
• regulation of calcium-mediated signaling
• L-ascorbic acid biosynthetic process

Molecular functions

• calcium ion binding
• enzyme regulator activity
• zinc ion binding
• gluconolactonase activity

Cellular components

• cytoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Six-bladed beta-propeller, TolB-like
• Senescence marker protein-30 (SMP-30)
• Regucalcin
• SMP-30/Gluconolactonase/LRE-like region
• SMP-30/Gluconolactonase/LRE-like region

KeywordsUniProt

• Calcium
• Cytoplasm
• Hydrolase
• Metal-binding
• Zinc