Seroatlas · Human Serome Atlas

R3HDM4

R3H domain-containing protein 4

Also known as: C19orf22, MGC16353, R3HD4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96D70
Gene: R3HDM4
Ensembl: ENSG00000198858
Chromosome: 19
Canonical length: 268 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable nucleic acid binding activity. Predicted to be located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

268 residues, UniProt reviewed canonical sequence.

>Q96D70|R3HDM4
     1  MVALENPECG PEAAEGTPGG RRLLPLPSCL PALASSQVKR LSASRRKQHF INQAVRNSDL
    61  VPKAKGRKSL QRLENTQYLL TLLETDGGLP GLEDGDLAPP ASPGIFAEAC NNATYVEVWN
   121  DFMNRSGEEQ ERVLRYLEDE GRSKARRRGP GRGEDRRRED PAYTPRECFQ RISRRLRAVL
   181  KRSRIPMETL ETWEERLLRF FSVSPQAVYT AMLDNSFERL LLHAVCQYMD LISASADLEG
   241  KRQMKVSNRH LDFLPPGLLL SAYLEQHS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against R3HDM4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 93 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 93 nTPM
spleen: 92 nTPM
esophagus: 81 nTPM
skin: 57 nTPM
lung: 55 nTPM
skeletal muscle: 54 nTPM

Single-cell type

neutrophils: 604 nCPM
platelets: 567 nCPM
erythrocytes: 216 nCPM
esophageal apical cells: 182 nCPM
megakaryocytes: 142 nCPM
neutrophil progenitors: 100 nCPM

Immune cell

neutrophil: 589 nTPM
basophil: 118 nTPM
eosinophil: 111 nTPM
T-reg: 49 nTPM
memory B-cell: 39 nTPM
memory CD4 T-cell: 30 nTPM

Brain region

white matter: 60 nTPM
cerebral cortex: 54 nTPM
cerebellum: 54 nTPM
amygdala: 53 nTPM
medulla oblongata: 50 nTPM
hippocampal formation: 48 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.08
gnomAD pLI: 0
gnomAD missense Z: -0.53
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

nucleic acid binding

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

R3H domain
R3H domain superfamily
R3H domain
R3H-associated N-terminal domain
R3H domain-containing protein 4
R3H-associated N-terminal domain

KeywordsUniProt

Nucleus

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads R3HDM4 as an antibody target. Whether an autoantibody or antibody against R3HDM4 could matter depends on whether native R3HDM4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

R3HDM4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label R3HDM4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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